Translational Research in Serious Human Diseases

0536629 - ÚŽFG 2021 RIV CZ eng M - Část monografie knihy
Vaškovičová, Michaela - Šolc, Petr
Laser microirradiation as a tool for DNA damage response studies.
Translational Research in Serious Human Diseases. Praha: Academia, 2020 - (Kello, M.; Strnadel, J.; Klempir, J.; Roth, J.; Myslivcová-Fučíková, A.; Hansíková, H.; Kozák, I.), s. 257-264. ISBN 978-80-200-3158-7
Grant CEP: GA MŠMT(CZ) LO1609
Institucionální podpora: RVO:67985904
Klíčová slova: laser microirradiation * DNA damage response * double-stranded DNA breaks
Obor OECD: Cell biology

Double-strand DNA breaks (DSBs) are considered as the most hazardous type of DNA lesions. Recognition of DSBs, temporal cell cycle arrest and activation of repair pathways are crucial steps in response to DNA damage. Errors in any of these steps can lead to adverse consequences affecting genome integrity, e.g. chromosomal aberrations or generation of mutations. The genomic instability caused by severe endpoints of DSBs processing can lead to cell death or development of cancer. Therefore, it is crucial to study all aspects of DNA damage response in cells.
In the context of studying DNA damage response in cells, DSBs are typically induced either by γ-irradiation or by UV light. DSBs can be also induced by radiomimetic drugs, such as Neocarzinostatin. However, these techniques have several limitations, e.g. inability to induced DSBs only in a specific region of nucleus. Therefore, the laser UV microirradiation technique combined with confocal microscopy was developed to study the DNA damage response in living cells.
Trvalý link: http://hdl.handle.net/11104/0314374