Translational Research in Serious Human Diseases

0536610 - ÚŽFG 2021 RIV CZ eng M - Část monografie knihy
Horák, Vratislav - Palánová, Anna - Kupcová Skalníková, Helena
Melanoma progression and spontaneous regression in the Melanoma-bearing Libechov Minipig (MeLiM) model.
Translational Research in Serious Human Diseases. Praha: Academia, 2020 - (Kello, M.; Strnadel, J.; Klempir, J.; Roth, J.; Myslivcová-Fučíková, A.; Hansíková, H.; Kozák, I.), s. 187-200. ISBN 978-80-200-3158-7
Grant CEP: GA MŠMT(CZ) LO1609; GA MŠMT(CZ) EF16_019/0000785
Institucionální podpora: RVO:67985904
Klíčová slova: melanoma * swine MeLiM model * melanoma progression
Obor OECD: Oncology

Cutaneous melanoma is a malignant tumour arising by neoplastic transformation of melanocytes, normal skin melanin-containing cells. It is the most dangerous skin cancer with worldwide incidence in Caucasian population increasing during the last decades. Despite present successes in the melanoma treatment using immune checkpoint inhibitors, its therapy in metastatic stage is still unsatisfactory and new approaches need to be sought. The Melanoma-bearing Libechov Minipig (MeLiM) is a unique model of hereditary metastatic melanoma which shows histopathological, immunohistochemical, biochemical, haematological, and genetic similarities with the human melanoma. Long-term monitoring and detailed analyses of affected animals allow identifying genes and understanding the biological processes associated with the melanoma development and progression as well as its spontaneous regression. In addition, new techniques for melanoma treatment can be tested using MeLiM piglets with progressing melanoma. Tumour devitalization (surgical ischaemization) was successfully utilized in animals with metastatic melanoma and multiple cutaneous
tumours. Increased expression of heat shock proteins HSP70 and gp96 in the devitalized melanoma followed by increased population of tumour infiltrating lymphocytes in all untreated melanomas and generalized destruction of melanoma cells in all cancer deposits including organ metastases were observed in the treated pigs. They were in long-term remission without relapse. MeLiM represents a valuable large animal model to study melanoma regression and new methods for melanoma treatment.
Trvalý link: http://hdl.handle.net/11104/0314363