Age-related differences in the translational landscape of mammalian oocytes

0536356 - ÚŽFG 2021 RIV US eng J - Článek v odborném periodiku
del Llano, Edgar - Mašek, T. - Gahurová, Lenka - Pospíšek, M. - Končická, Markéta - Jindrová, Anna - Jansová, Denisa - Iyyappan, Rajan - Roučová, K. - Bruce, A. W. - Kubelka, Michal - Šušor, Andrej
Age-related differences in the translational landscape of mammalian oocytes.
Aging Cell. Roč. 19, č. 10 (2020), č. článku e13231. ISSN 1474-9718. E-ISSN 1474-9726
Grant CEP: GA ČR(CZ) GA19-13491S
Institucionální podpora: RVO:67985904
Klíčová slova: aging * cytokinesis * meiosis * oocyte * translation
Obor OECD: Biology (theoretical, mathematical, thermal, cryobiology, biological rhythm), Evolutionary biology
Impakt faktor: 9.304, rok: 2020
Způsob publikování: Open access
https://onlinelibrary.wiley.com/doi/full/10.1111/acel.13231

Increasing maternal age in mammals is associated with poorer oocyte quality, involving higher aneuploidy rates and decreased developmental competence. Prior to resumption of meiosis, fully developed mammalian oocytes become transcriptionally silent until the onset of zygotic genome activation. Therefore, meiotic progression and early embryogenesis are driven largely by translational utilization of previously synthesized mRNAs. We report that genome-wide translatome profiling reveals considerable numbers of transcripts that are differentially translated in oocytes obtained from aged compared to young females. Additionally, we show that a number of aberrantly translated mRNAs in oocytes from aged females are associated with cell cycle. Indeed, we demonstrate that four specific maternal age-related transcripts (Sgk1,Castor1,AireandEg5) with differential translation rates encode factors that are associated with the newly forming meiotic spindle. Moreover, we report substantial defects in chromosome alignment and cytokinesis in the oocytes of young females, in which candidate CASTOR1 and SGK1 protein levels or activity are experimentally altered. Our findings indicate that improper translation of specific proteins at the onset of meiosis contributes to increased chromosome segregation problems associated with female ageing.
Trvalý link: http://hdl.handle.net/11104/0314135