Unique members of the adipokinetic hormone family in butterflies and moths (Insecta, Lepidoptera)

0536352 - BC 2021 RIV CH eng J - Článek v odborném periodiku
Marco, H. G. - Šimek, Petr - Gäde, G.
Unique members of the adipokinetic hormone family in butterflies and moths (Insecta, Lepidoptera).
Frontiers in Physiology. Roč. 11, DEC 17 (2020), č. článku 614552. ISSN 1664-042X. E-ISSN 1664-042X
Grant CEP: GA ČR(CZ) GA17-22276S
Institucionální podpora: RVO:60077344
Klíčová slova: butterflies and moths * Lepidoptera * adipokinetic hormone
Obor OECD: Biochemistry and molecular biology
Impakt faktor: 4.566, rok: 2020
Způsob publikování: Open access
https://www.frontiersin.org/articles/10.3389/fphys.2020.614552/pdf

Lepidoptera is amongst one of the four most speciose insect orders and ecologically very successful because of their ability to fly. Insect flight is always aerobic and exacts
a high metabolic demand on the animal. A family of structurally related neuropeptides, generically referred to as adipokinetic hormones (AKHs), play a key role in triggering
the release of readily utilizable fuel metabolites into the hemolymph from the storage forms in the fat body. We used mass spectrometry to elucidate AKH sequences
from 34 species of Lepidoptera and searched the literature and publicly available databases to compile (in a phylogenetic context) a comprehensive list of all Lepidoptera sequences published/predicted from a total of 76 species. We then used the resulting set of 15 biochemically characterized AKHs in a physiological assay that measures
lipid or carbohydrate mobilization in three different lepidopteran species to learn about the functional cross-activity (receptor-ligand interactions) amongst the different
butterfly/moth families. Our results include novel peptide structures, demonstrate structural diversity, phylogenetic trends in peptide distribution and order-specificity
of Lepidoptera AKHs. There is almost an equal occurrence of octa-, nona-, and decapeptides, with an unparalleled emphasis on nonapeptides than in any insect order.
Primitive species make Peram-CAH-II, an octapeptide found also in other orders, the lepidopteran signature peptide is Manse-AKH. Not all of the 15 tested AKHs are active
in Pieris brassicae, this provides insight into structure-activity specificity and could be useful for further investigations into possible biorational insecticide development.
Trvalý link: http://hdl.handle.net/11104/0316034