In Cell NMR Spectroscopy: Investigation of G-Quadruplex Structures Inside Living Xenopus laevis Oocytes

0535922 - BFÚ 2021 RIV US eng J - Článek v odborném periodiku
Krafčíková, Michaela - Hansel-Hertsch, R. - Trantírek, Lukáš - Foldynová-Trantírková, Silvie
In Cell NMR Spectroscopy: Investigation of G-Quadruplex Structures Inside Living Xenopus laevis Oocytes.
Methods in Molecular Biology. Methods in Molecular Biology. Roč. 2035, č. 2019 (2019), s. 397-405. ISSN 1064-3745
Grant CEP: GA ČR GA17-12075S; GA MŠMT EF15_003/0000477
Institucionální podpora: RVO:68081707
Klíčová slova: extracts * rna * dna * In-cell NMR * Xenopus laevis oocyte
Obor OECD: Biochemistry and molecular biology
Způsob publikování: Open access s časovým embargem
https://link.springer.com/protocol/10.1007/978-1-4939-9666-7_25

G-quadruplexes are inherently polymorphic nucleic acid structures. Their folding topology depends on the nucleic acid primary sequence and on physical-chemical environmental factors. Hence, it remains unclear if a G-quadruplex topology determined in the test tube (in vitro) will also form in vivo. Characterization of G-quadruplexes in their native environment has been proposed as an efficient strategy to tackle this issue. So far, characterization of G-quadruplex structures in living cells has relied exclusively on the use of Xenopus laevis oocytes as a eukaryotic cell model system. Here, we describe the protocol for the preparation of X. laevis oocytes for studies of G-quadruplexes as well as other nucleic acids motifs under native conditions using in-cell NMR spectroscopy.
Trvalý link: http://hdl.handle.net/11104/0313799