Feeding on fungi: genomic and proteomic analysis of the enzymatic machinery of bacteria decomposing fungal biomass

0535741 - MBÚ 2021 RIV US eng J - Článek v odborném periodiku
Starke, Robert - Morais, Daniel - Větrovský, Tomáš - López-Mondejár, Rubén - Baldrian, Petr - Brabcová, Vendula
Feeding on fungi: genomic and proteomic analysis of the enzymatic machinery of bacteria decomposing fungal biomass.
Environmental Microbiology. Roč. 22, č. 11 (2020), s. 4604-4619. ISSN 1462-2912. E-ISSN 1462-2920
Grant CEP: GA ČR(CZ) GA17-20110S; GA MŠMT(CZ) ED1.1.00/02.0109
Institucionální podpora: RVO:61388971
Klíčová slova: cultivated mushrooms * ewingella-americana * n-acetylglucosamine * agaricus-bisporus
Obor OECD: Microbiology
Impakt faktor: 5.491, rok: 2020
Způsob publikování: Omezený přístup
https://sfamjournals.onlinelibrary.wiley.com/doi/abs/10.1111/1462-2920.15183

Dead fungal biomass is an abundant source of nutrition in both litter and soil of temperate forests largely decomposed by bacteria. Here, we have examined the utilization of dead fungal biomass by the five dominant bacteria isolated from thein situdecomposition of fungal mycelia using a multiOMIC approach. The genomes of the isolates encoded a broad suite of carbohydrate-active enzymes, peptidases and transporters. In the extracellular proteome, onlyEwingella americanaexpressed chitinases while the twoPseudomonasisolates attacked chitin by lytic chitin monooxygenase, deacetylation and deamination.Variovoraxsp. expressed enzymes acting on the side-chains of various glucans and the chitin backbone. Surprisingly, despite its genomic potential,Pedobactersp. did not produce extracellular proteins to decompose fungal mycelia but presumably feeds on simple substrates. The ecological roles of the five individual strains exhibited complementary features for a fast and efficient decomposition of dead fungal biomass by the entire bacterial community.
Trvalý link: http://hdl.handle.net/11104/0313686