Extended Set of GoldenBraid Compatible Vectors for Fast Assembly of Multigenic Constructs and Their Use to Create Geminiviral Expression Vectors

0535107 - ÚEB 2021 RIV CH eng J - Článek v odborném periodiku
Dušek, Jakub - Plchová, Helena - Čeřovská, Noemi - Pobořilová, Zuzana - Navrátil, Oldřich - Kratochvílová, Kateřina - Gunter, C. - Jacobs, R. - Hitzeroth, I. I. - Rybicki, E. P. - Moravec, Tomáš
Extended Set of GoldenBraid Compatible Vectors for Fast Assembly of Multigenic Constructs and Their Use to Create Geminiviral Expression Vectors.
Frontiers in Plant Science. Roč. 11, OCT 22 (2020), č. článku 522059. ISSN 1664-462X. E-ISSN 1664-462X
Grant CEP: GA ČR(CZ) GA15-10768S; GA MŠMT(CZ) EF16_019/0000738
Institucionální podpora: RVO:61389030
Klíčová slova: Agrobacterium tumefaciens * cloning * Nicotiana benthamiana * plant virus vector * synthetic biology * transient expression
Obor OECD: Biochemical research methods
Impakt faktor: 5.754, rok: 2020
Způsob publikování: Open access
http://doi.org/10.3389/fpls.2020.522059

Methods for simple and fast assembly of exchangeable standard DNA parts using Type II S restriction enzymes are becoming more and more popular in plant synthetic and molecular biology. These methods enable routine construction of large and complex multigene DNA structures. Two available frameworks emphasize either high cloning capacity (Modular Cloning, MoClo) or simplicity (GoldenBraid, GB). Here we present a set of novel α-level plasmids compatible with the GB convention that extend the ability of GB to rapidly assemble more complex genetic constructs, while maintaining compatibility with all existing GB parts as well as most MoClo parts and GB modules. With the use of our new plasmids, standard GB parts can be assembled into complex assemblies containing 1, 5, 10 and up to theoretically 50 units in each successive level of infinite loop assembly. Assembled DNA constructs can be also combined with conventional binary GB-assemblies (1, 2, 4, 8… units). We demonstrate the usefulness of our framework on single tube assembly of replicating plant expression constructs based on the geminivirus Bean yellow dwarf virus (BeYDV).
Trvalý link: http://hdl.handle.net/11104/0313211