Analysis of Chondroitin/Dermatan Sulphate Disaccharides Using High-Performance Liquid Chromatography

0534343 - FGÚ 2021 RIV CH eng J - Článek v odborném periodiku
Mikšík, Ivan - Kubinová, Šárka - Morvan, Marine - Výborný, Karel - Tatar, A. - Král, V. - Záruba, K. - Sýkora, D.
Analysis of Chondroitin/Dermatan Sulphate Disaccharides Using High-Performance Liquid Chromatography.
Separations. Roč. 7, č. 3 (2020), č. článku 49. E-ISSN 2297-8739
Grant CEP: GA ČR(CZ) GA18-02597S; GA MŠMT(CZ) EF15_003/0000419
Institucionální podpora: RVO:67985823 ; RVO:68378041
Klíčová slova: chondroitin * disaccharides * dermatan sulphates
Obor OECD: Analytical chemistry; Neurosciences (including psychophysiology (UEM-P)
Impakt faktor: 2.777, rok: 2020
Způsob publikování: Open access
https://www.mdpi.com/2297-8739/7/3/49

Chondroitin sulphates belong to a group of naturally occurring glycosaminoglycans and play a role in many physiological processes including ageing and the effects of various diseases. Research into chondroitin sulphates has found that the most important analytes are 4- and 6-sulphated disaccharides. We developed an HPLC method for the separation and quantification of underivatized chondroitin/dermatan sulphates-unsaturated disaccharides (4- and 6-sulphated disaccharides). This method is based on the separation of disaccharides by amido as well as amino columns under acidic conditions. These columns enabled the successful separation of 4- and 6-sulphated disaccharides using 50 (amido column) and 25 mmol/L (amino column) phosphate buffer, pH 4.25 (detection at 230 nm), at retention times of less than 10 min. The limit of quantification was 0.5 mu g/mL. The applicability of this method was demonstrated through analysis of unsaturated disaccharides produced from the enzymatic digestion of chondroitin/dermatan sulphates of the solubilized extracellular matrix produced from porcine urinary bladder and human umbilical cord.
Trvalý link: http://hdl.handle.net/11104/0312560