Versatile bioconjugation strategies of PEG-modified upconversion nanoparticles for bioanalytical applications

0534143 - ÚMCH 2021 RIV US eng J - Článek v odborném periodiku
Kostiv, Uliana - Farka, Z. - Mickert, M. J. - Gorris, H. H. - Velychkivska, Nadiia - Pop-Georgievski, Ognen - Pastucha, M. - Odstrčilíková, E. - Skládal, P. - Horák, Daniel
Versatile bioconjugation strategies of PEG-modified upconversion nanoparticles for bioanalytical applications.
Biomacromolecules. Roč. 21, č. 11 (2020), s. 4502-4513. ISSN 1525-7797. E-ISSN 1526-4602
Grant CEP: GA ČR(CZ) GA19-00676S; GA MŠMT(CZ) LTAB19011; GA ČR(CZ) GA20-07313S
Institucionální podpora: RVO:61389013
Klíčová slova: upconversion nanoparticles * PEG-neridronate * bioconjugation
Obor OECD: Polymer science
Impakt faktor: 6.988, rok: 2020
Způsob publikování: Omezený přístup
https://pubs.acs.org/doi/10.1021/acs.biomac.0c00459

Lanthanide-doped upconversion nanoparticles (UCNPs) display highly beneficial photophysical features for background-free bioimaging and bioanalysis. They are instable in high ionic strength buffers, have no functional groups, and are nonspecifically interacting. Here, we have prepared NIR-excitable UCNPs that are long-term colloidally stable in buffered media and possess functional groups. Heterobifunctional poly(ethylene glycol) (PEG) linkers bearing neridronate and alkyne or maleimide were attached to UCNPs via a ligand exchange. Streptavidin (SA)-conjugates were prepared by click reaction of UCNP@PEG-alkyne and SA-azide. Antihuman serum albumin pAbF antibody was modified with azide groups and conjugated to UCNP@PEG-alkyne via click reaction. Alternatively, the antibody, after mild reduction of its disulfide bonds, was conjugated to UCNP@PEG-maleimide. We employed these nanoconjugates as labels for an upconversion-linked immunosorbent assay. SA-based labels achieved the lowest LOD of 0.17 ng/mL for the target albumin, which was superior compared to a fluorescence immunoassay (LOD 0.59 ng/mL) or an enzyme-linked immunoassay (LOD 0.56 ng/mL).
Trvalý link: http://hdl.handle.net/11104/0312883