Phospholipase D alpha 1 mediates thehigh-Mg(2)(+)stress response partially through regulation of K(+)homeostasis

0533491 - ÚEB 2021 RIV GB eng J - Článek v odborném periodiku
Kocourková, Daniela - Krčková, Zuzana - Pejchar, Přemysl - Kroumanová, Kristýna - Podmanická, Tereza - Daněk, Michal - Martinec, Jan
Phospholipase D alpha 1 mediates thehigh-Mg(2)(+)stress response partially through regulation of K(+)homeostasis.
Plant Cell and Environment. Roč. 43, č. 10 (2020), s. 2460-2475. ISSN 0140-7791. E-ISSN 1365-3040
Grant CEP: GA ČR(CZ) GA17-00522S; GA MŠMT(CZ) EF16_019/0000738
Institucionální podpora: RVO:61389030
Klíčová slova: Arabidopsis thaliana * cipk9 * hak5 * high magnesium * ion homeostasis * phospholipase D * potassium
Obor OECD: Biochemical research methods
Impakt faktor: 7.228, rok: 2020
Způsob publikování: Open access
http://doi.org/10.1111/pce.13831

Intracellular levels of Mg2+ are tightly regulated, as Mg2+ deficiency or excess affects normal plant growth and development. In Arabidopsis, we determined that phospholipase Dα1 (PLDα1) is involved in the stress response to high-magnesium conditions. The T-DNA insertion mutant pldα1 is hypersensitive to increased concentrations of magnesium, exhibiting reduced primary root length and fresh weight. PLDα1 activity increases rapidly after high-Mg2+ treatment, and this increase was found to be dose dependent. Two lines harbouring mutations in the HKD motif, which is essential for PLDα1 activity, displayed the same high-Mg2+ hypersensitivity of pldα1 plants. Moreover, we show that high concentrations of Mg2+ disrupt K+ homeostasis, and that transcription of K+ homeostasis-related genes CIPK9 and HAK5 is impaired in pldα1. Additionally, we found that the akt1, hak5 double mutant is hypersensitive to high-Mg2+. We conclude that in Arabidopsis, the enzyme activity of PLDα1 is vital in the response to high-Mg2+ conditions, and that PLDα1 mediates this response partially through regulation of K+ homeostasis.
Trvalý link: http://hdl.handle.net/11104/0311868