Cytokinin fluoroprobe reveals multiple sites of cytokinin perception at plasma membrane and endoplasmic reticulum

0532925 - ÚEB 2021 RIV GB eng J - Článek v odborném periodiku
Kubiasová, K. - Montesinos, J. C. - Šamajová, O. - Nisler, Jaroslav - Mik, V. - Semerádová, H. - Plíhalová, Lucie - Novák, Ondřej - Marhavý, P. - Cavallari, N. - Zalabák, D. - Berka, K. - Doležal, Karel - Galuszka, P. - Šamaj, J. - Strnad, Miroslav - Benková, E. - Plíhal, Ondřej - Spíchal, L.
Cytokinin fluoroprobe reveals multiple sites of cytokinin perception at plasma membrane and endoplasmic reticulum.
Nature Communications. Roč. 11, č. 1 (2020), č. článku 4285. E-ISSN 2041-1723
Grant CEP: GA MŠMT(CZ) EF16_019/0000827; GA ČR(CZ) GA16-04184S
Institucionální podpora: RVO:61389030
Klíčová slova: LIGAND-BINDING PROPERTIES * ARABIDOPSIS-THALIANA * HISTIDINE KINASE
Obor OECD: Biochemistry and molecular biology
Impakt faktor: 14.919, rok: 2020
Způsob publikování: Open access
http://doi.org/10.1038/s41467-020-17949-0

Plant hormone cytokinins are perceived by a subfamily of sensor histidine kinases (HKs), which via a two-component phosphorelay cascade activate transcriptional responses in the nucleus. Subcellular localization of the receptors proposed the endoplasmic reticulum (ER) membrane as a principal cytokinin perception site, while study of cytokinin transport pointed to the plasma membrane (PM)-mediated cytokinin signalling. Here, by detailed monitoring of subcellular localizations of the fluorescently labelled natural cytokinin probe and the receptor ARABIDOPSIS HISTIDINE KINASE 4 (CRE1/AHK4) fused to GFP reporter, we show that pools of the ER-located cytokinin receptors can enter the secretory pathway and reach the PM in cells of the root apical meristem, and the cell plate of dividing meristematic cells. Brefeldin A (BFA) experiments revealed vesicular recycling of the receptor and its accumulation in BFA compartments. We provide a revised view on cytokinin signalling and the possibility of multiple sites of perception at PM and ER.
Trvalý link: http://hdl.handle.net/11104/0311304