The structural basis for the selectivity of sulfonamido dicarbaboranes toward cancer-associated carbonic anhydrase IX

0532715 - ÚOCHB 2021 RIV GB eng J - Článek v odborném periodiku
Kugler, Michael - Holub, Josef - Brynda, Jiří - Pospíšilová, Klára - El Anwar, Suzan - Bavol, Dmytro - Havránek, M. - Král, V. - Fábry, M. - Grüner, Bohumír - Řezáčová, Pavlína
The structural basis for the selectivity of sulfonamido dicarbaboranes toward cancer-associated carbonic anhydrase IX.
Journal of Enzyme Inhibition and Medicinal Chemistry. Roč. 35, č. 1 (2020), s. 1800-1810. ISSN 1475-6366. E-ISSN 1475-6374
Grant CEP: GA ČR(CZ) GA18-27648S; GA TA ČR(CZ) TE01020028; GA MŠMT(CZ) EF16_019/0000729
Grant ostatní: AV ČR(CZ) L200321851
Program: Program podpory perspektivníchlidských zdrojů - postdoktorandů
Institucionální podpora: RVO:61388963 ; RVO:61388980
Klíčová slova: carbonic anhydrase IX * carbone * enzyme inhibitors * sulfonamide * structure-activity relationship
Obor OECD: Biochemistry and molecular biology; Inorganic and nuclear chemistry (UACH-T)
Impakt faktor: 5.051, rok: 2020
Způsob publikování: Open access
https://doi.org/10.1080/14756366.2020.1816996

Human carbonic anhydrase IX (CA IX), a protein specifically expressed on the surface of solid tumour cells, represents a validated target both for anticancer therapy and diagnostics. We recently identified sulfonamide dicarbaboranes as promising inhibitors of CA IX with favourable activities both in vitro and in vivo. To explain their selectivity and potency, we performed detailed X-ray structural analysis of their interactions within the active sites of CA IX and CA II. Series of compounds bearing various aliphatic linkers between the dicarbaborane cluster and sulfonamide group were examined. Preferential binding towards the hydrophobic part of the active site cavity was observed. Selectivity towards CA IX lies in the shape complementarity of the dicarbaborane cluster with a specific CA IX hydrophobic patch containing V131 residue. The bulky side chain of F131 residue in CA II alters the shape of the catalytic cavity, disrupting favourable interactions of the spherical dicarbaborane cluster.
Trvalý link: http://hdl.handle.net/11104/0311123