A novel strigolactone-miR156 module controls stomatal behaviour during drought recovery

0532595 - ÚEB 2021 RIV GB eng J - Článek v odborném periodiku
Visentin, I. - Pagliarani, C. - Deva, E. - Caracci, A. - Turečková, Veronika - Novák, Ondřej - Lovisolo, C. - Schubert, A. - Cardinale, F.
A novel strigolactone-miR156 module controls stomatal behaviour during drought recovery.
Plant Cell and Environment. Roč. 43, č. 7 (2020), s. 1613-1624. ISSN 0140-7791. E-ISSN 1365-3040
Grant CEP: GA MŠMT(CZ) LO1204
Institucionální podpora: RVO:61389030
Klíčová slova: abscisic acid (ABA) * after-effect of drought * hormone signalling * osmotic stress * Solanum lycopersicum * stomata * stress-responsive microRNA
Obor OECD: Plant sciences, botany
Impakt faktor: 7.228, rok: 2020
Způsob publikování: Open access
http://doi.org/10.1111/pce.13758

miR156 is a conserved microRNA whose role and induction mechanisms under stress are poorly known. Strigolactones are phytohormones needed in shoots for drought acclimation. They promote stomatal closure ABA-dependently and independently, however, downstream effectors for the former have not been identified. Linkage between miR156 and strigolactones under stress has not been reported. We compared ABA accumulation and sensitivity as well as performances of wt and miR156-overexpressing (miR156-oe) tomato plants during drought. We also quantified miR156 levels in wt, strigolactone-depleted and strigolactone-treated plants, exposed to drought stress. Under irrigated conditions, miR156 overexpression and strigolactone treatment led to lower stomatal conductance and higher ABA sensitivity. Exogenous strigolactones were sufficient for miR156 accumulation in leaves, while endogenous strigolactones were required for miR156 induction by drought. The “after-effect” of drought, by which stomata do not completely re-open after rewatering, was enhanced by both strigolactones and miR156. The transcript profiles of several miR156 targets were altered in strigolactone-depleted plants. Our results show that strigolactones act as a molecular link between drought and miR156 in tomato, and identify miR156 as a mediator of ABA-dependent effect of strigolactones on the after-effect of drought on stomata. Thus, we provide insights into both strigolactone and miR156 action on stomata.
Trvalý link: http://hdl.handle.net/11104/0311038