Novel versatile 3D bio-scaffold made of natural biocompatible hagfish exudate for tissue growth and organoid modeling

0532432 - MBÚ 2021 RIV NL eng J - Článek v odborném periodiku
Dastjerdi, M.B. - Amini, A. - Nazari, M. - Cheng, Ch. - Benson, Veronika - Gholami, A. - Ghasemi, Y.
Novel versatile 3D bio-scaffold made of natural biocompatible hagfish exudate for tissue growth and organoid modeling.
International Journal of Biological Macromolecules. Roč. 158, SEP (2020), s. 894-902. ISSN 0141-8130. E-ISSN 1879-0003
Institucionální podpora: RVO:61388971
Klíčová slova: 3-dimensional cell-culture * hydrogel scaffolds * aqueous-solution * removal
Obor OECD: Microbiology
Impakt faktor: 6.953, rok: 2020
Způsob publikování: Omezený přístup
https://www.sciencedirect.com/science/article/pii/S0141813020331718?via%3Dihub

Hagfish exudate is a natural biological macromolecule made of keratin intermediate filament protein skeins and mucin vesicles. Here, we successfully examined this remarkable biomaterial as a substrate for three-dimensional (3D) cell culturing purposes. After the sterilization with chloroform vapor, Dulbecco's modified eagle medium was mixed with the exudate to rupture the vesicles and skeins, a highly soft, adherent, fibrous and biocompatible hydrogel was formed. A variety of cells, including Hela-FUCCI, NMuMG-FUCCI, 10T1/2 and C2C12, was cultured on the hagfish exudate. A remarkable 3D growth by-2.5 folds after day 3,-5 folds after day 5,-10 folds after day 7 and15 folds after day 14 were seen compared to day one of culturing in the hagfish exudate scaffold. In addition, the phase contrast, fluorescent and confocal microscopy observations confirmed the organoid shape formation within the three-week culture. The viability of cells was almost 100% indicating the great in vitro and in vivo potential of this exceptional biomaterial with no cytotoxic effect. (c) 2020 Elsevier B.V. All rights reserved.
Trvalý link: http://hdl.handle.net/11104/0310924