DNA Methylation Profiles in a Group of Workers Occupationally Exposed to Nanoparticles

0532304 - ÚEM 2021 RIV CH eng J - Článek v odborném periodiku
Rössnerová, Andrea - Hoňková, Kateřina - Pelclová, D. - Ždímal, Vladimír - Hubáček, J. A. - Chvojková, Irena - Vrbová, Kristýna - Rössner ml., Pavel - Topinka, Jan - Vlčková, Š. - Fenclová, Z. - Lischková, L. - Klusáčková, P. - Schwarz, Jaroslav - Ondráček, Jakub - Ondráčková, Lucie - Koštejn, Martin - Kléma, J. - Dvořáčková, Š.
DNA Methylation Profiles in a Group of Workers Occupationally Exposed to Nanoparticles.
International Journal of Molecular Sciences. Roč. 21, č. 7 (2020), č. článku 2420. E-ISSN 1422-0067
Grant CEP: GA ČR(CZ) GA18-02079S
Institucionální podpora: RVO:68378041 ; RVO:67985858
Klíčová slova: 850K microarray * CpG sites * DNA methylation
Obor OECD: Occupational health; Public and environmental health (UCHP-M)
Impakt faktor: 5.924, rok: 2020
Způsob publikování: Open access
https://www.mdpi.com/1422-0067/21/7/2420

The risk of exposure to nanoparticles (NPs) has rapidly increased during the last decade due to the vast use of nanomaterials (NMs) in many areas of human life. Despite this fact, human biomonitoring studies focused on the effect of NP exposure on DNA alterations are still rare. Furthermore, there are virtually no epigenetic data available. In this study, we investigated global and gene-specific DNA methylation profiles in a group of 20 long-term (mean 14.5 years) exposed, nanocomposite, research workers and in 20 controls. Both groups were sampled twice/day (pre-shift and post-shift) in September 2018. We applied Infinium Methylation Assay, using the Infinium MethylationEPIC BeadChips with more than 850,000 CpG loci, for identification of the DNA methylation pattern in the studied groups. Aerosol exposure monitoring, including two nanosized fractions, was also performed as proof of acute NP exposure. The obtained array data showed significant differences in methylation between the exposed and control groups related to long-term exposure, specifically 341 CpG loci were hypomethylated and 364 hypermethylated. The most significant CpG differences were mainly detected in genes involved in lipid metabolism, the immune system, lung functions, signaling pathways, cancer development and xenobiotic detoxification. In contrast, short-term acute NP exposure was not accompanied by DNA methylation changes. In summary, long-term (years) exposure to NP is associated with DNA epigenetic alterations.
Trvalý link: http://hdl.handle.net/11104/0312571