Structural characteristics and biological effects of exopolysaccharide produced by cyanobacterium Nostoc sp

0532233 - BÚ 2021 RIV GB eng J - Článek v odborném periodiku
Uhliariková, I. - Šutovská, M. - Barboríková, J. - Molitorisová, M. - Kim, H. J. - Park, Y. I. - Matulová, M. - Lukavský, Jaromír - Hromadková, Z. - Capek, P.
Structural characteristics and biological effects of exopolysaccharide produced by cyanobacterium Nostoc sp.
International Journal of Biological Macromolecules. Roč. 160, OCT 1 (2020), s. 364-371. ISSN 0141-8130. E-ISSN 1879-0003
Grant CEP: GA TA ČR TE01020080
Institucionální podpora: RVO:67985939
Klíčová slova: Cyanobacteria * Extracellular polysaccharide * Biological activities
Obor OECD: Biochemistry and molecular biology
Impakt faktor: 6.953, rok: 2020
Způsob publikování: Omezený přístup

Complex structure of cyanobacterium Nostoc sp. exopolysaccharide (EPS), with apparent molecular weight 214 × 103 g/mol, can be deduced from its composition. Chemical and NMR analyses found four dominant sugarmonomers, namely (1→ 4)-linked α-L-arabinopyranose, β-D-glucopyranose, β-D-xylopyranose and (1→ 3)-linked β-D-mannopyranose, two different uronic acids and a lactyl group, with (1→4,6)-linked β-D-glucopyranose as the only branch point suggest a complex structure of this polymer. The dominant uronic acid is α-linked, but it remained unidentified. β-D-Glucuronic acid was present in lower amount. Their position as well as that of lactyl remained undetermined too. Different doses of orally administered EPS in guinea pigs evoked a significant decrease in cough effort and a decrease in airway reactivity. The antitussive efficacy and bronchodilator effect of higher doses of EPS were found to be similar to that of the antitussive drug codeine and the antiasthmatic salbutamol. Without significant cytotoxicity on the RAW264.7 cells, EPS stimulated themacrophage cells to produce pro-inflammatory cytokines tumor necrosis factor-α (TNF-α) and interleukin-6 (IL-6), and prostaglandins (PGs) and nitric oxide (NO) via induction of COX-2 and iNOS expression, respectively, suggesting that this biopolymer potentiates an early innate immune response and can therefore be used as a new immune modulator.
Trvalý link: http://hdl.handle.net/11104/0310806