Počet záznamů: 1
Nucleotide‐Bearing Benzylidene‐Tetrahydroxanthylium Near‐IR Fluorophore for Sensing DNA Replication, Secondary Structures and Interactions
- 1.0532176 - ÚOCHB 2021 RIV DE eng J - Článek v odborném periodiku
Kuba, Miroslav - Kraus, Tomáš - Pohl, Radek - Hocek, Michal
Nucleotide‐Bearing Benzylidene‐Tetrahydroxanthylium Near‐IR Fluorophore for Sensing DNA Replication, Secondary Structures and Interactions.
Chemistry - A European Journal. Roč. 26, č. 52 (2020), s. 11950-11954. ISSN 0947-6539. E-ISSN 1521-3765
Grant CEP: GA ČR(CZ) GA17-14791S; GA MŠMT(CZ) EF16_019/0000729
Grant ostatní: AV ČR(CZ) AP1501
Program: Akademická prémie - Praemium Academiae
Institucionální podpora: RVO:61388963
Klíčová slova: DNA * fluorescence * nucleotides * real-time PCR
Obor OECD: Organic chemistry
Impakt faktor: 5.236, rok: 2020
Způsob publikování: Open access
https://doi.org/10.1002/chem.202003192
Thymidine triphosphate bearing benzylidene‐tetrahydroxanthylium near‐IR fluorophore linked to the 5‐methyl group via triazole was synthesized through the CuAAC reaction and was used for polymerase synthesis of labelled DNA probes. The fluorophore lights up upon incorporation to DNA (up to 348‐times) presumably due to interactions in major groove and the fluorescence further increases in the single‐stranded oligonucleotide. The labelled dsDNA senses binding of small molecules and proteins by a strong decrease of fluorescence. The nucleotide was used as a light‐up building block in real‐time PCR for detection of SARS‐CoV‐2 virus.
Trvalý link: http://hdl.handle.net/11104/0310762
Počet záznamů: 1