Osteogenic impact of pro-apoptotic caspase inhibitors in MC3T3-E1 cells

0532058 - ÚŽFG 2021 RIV GB eng J - Článek v odborném periodiku
Kratochvílová, Adéla - Veselá, Barbora - Ledvina, Vojtěch - Švandová, Eva - Klepárník, Karel - Dadáková, Kateřina - Beneš, P. - Matalová, Eva
Osteogenic impact of pro-apoptotic caspase inhibitors in MC3T3-E1 cells.
Scientific Reports. Roč. 10, č. 1 (2020), č. článku 7489. ISSN 2045-2322. E-ISSN 2045-2322
Grant CEP: GA ČR(CZ) GA19-14727S; GA ČR(CZ) GA20-00726S
Institucionální podpora: RVO:67985904 ; RVO:68081715
Klíčová slova: caspase * osteoblastic cells
Obor OECD: Developmental biology; Analytical chemistry (UIACH-O)
Impakt faktor: 4.380, rok: 2020
Způsob publikování: Open access
https://www.nature.com/articles/s41598-020-64294-9

Caspases are proteases traditionally associated with inflammation and cell death. Recently, they have also been shown to modulate cell proliferation and differentiation. The aim of the current research was to search for osteogenic molecules affected by caspase inhibition and to specify the individual caspases critical for these effects with a focus on proapoptotic caspases: caspase-2, -3, -6, -7, -8 and -9. Along with osteocalcin (Ocn), general caspase inhibition significantly decreased the expression of the Phex gene in differentiated MC3T3-E1 cells. The inhibition of individual caspases indicated that caspase-8 is a major contributor to the modification of Ocn and Phex expression. Caspase-2 and-6 had effects on Ocn and caspase-6 had an effect on Phex. These data confirm and expand the current knowledge about the nonapoptotic roles of caspases and the effect of their pharmacological inhibition on the osteogenic potential of osteoblastic cells.
Trvalý link: http://hdl.handle.net/11104/0310667