Glucose-Induced Expression of DAPIT in Pancreatic beta-Cells

0531801 - FGÚ 2021 RIV CH eng J - Článek v odborném periodiku
Leguina-Ruzzi, Alberto A. - Vodičková, Anežka - Holendová, Blanka - Pavluch, Vojtěch - Tauber, Jan - Engstová, Hana - Dlasková, Andrea - Ježek, Petr
Glucose-Induced Expression of DAPIT in Pancreatic beta-Cells.
Biomolecules. Roč. 10, č. 7 (2020), č. článku 1026. E-ISSN 2218-273X
Grant CEP: GA ČR(CZ) GA20-00408S
Institucionální podpora: RVO:67985823
Klíčová slova: mitochondria * USMG5/DAPIT * glucose-stimulated insulin secretion * glucose-induced expression * membrane subunits of ATP synthase * ATP synthase oligomers mitochondrial cristae morphology
Obor OECD: Endocrinology and metabolism (including diabetes, hormones)
Impakt faktor: 4.879, rok: 2020
Způsob publikování: Open access
https://www.mdpi.com/2218-273X/10/7/1026

Transcript levels for selected ATP synthase membrane F-O-subunits-including DAPIT-in INS-1E cells were found to be sensitive to lowering glucose down from 11 mM, in which these cells are routinely cultured. Depending on conditions, the diminished mRNA levels recovered when glucose was restored to 11 mM, or were elevated during further 120 min incubations with 20-mM glucose. Asking whether DAPIT expression may be elevated by hyperglycemia in vivo, we studied mice with hyaluronic acid implants delivering glucose for up to 14 days. Such continuous two-week glucose stimulations in mice increased DAPIT mRNA by >5-fold in isolated pancreatic islets (ATP synthase F-1 alpha mRNA by 1.5-fold). In INS-1E cells, the glucose-induced ATP increment vanished with DAPIT silencing (6% of ATP rise), likewise a portion of the mtDNA-copy number increment. With 20 and 11-mM glucose the phosphorylating/non-phosphorylating respiration rate ratio diminished to similar to 70% and 96%, respectively, upon DAPIT silencing, whereas net GSIS rates accounted for 80% and 90% in USMG5/DAPIT-deficient cells. Consequently, the sufficient DAPIT expression and complete ATP synthase assembly is required for maximum ATP synthesis and mitochondrial biogenesis, but not for insulin secretion as such. Elevated DAPIT expression at high glucose further increases the ATP synthesis efficiency.
Trvalý link: http://hdl.handle.net/11104/0310418