Počet záznamů: 1
Cytogenetic Characterization of Seven Novel satDNA Markers in Two Species of Spined Loaches (Cobitis) and Their Clonal Hybrids
- 1.0531710 - ÚŽFG 2021 RIV CH eng J - Článek v odborném periodiku
Marta, Anatolie - Didukh, Dmitrij - Bartoš, Oldřich - Majtánová, Zuzana - Janko, Karel
Cytogenetic Characterization of Seven Novel satDNA Markers in Two Species of Spined Loaches (Cobitis) and Their Clonal Hybrids.
Genes. Roč. 11, č. 6 (2020), č. článku 647. E-ISSN 2073-4425
Grant CEP: GA ČR(CZ) GA19-21552S; GA MŠMT EF15_003/0000460
Institucionální podpora: RVO:67985904
Klíčová slova: clonal vertebrates * hybridization * satellite DNA * FISH * mitotic and lampbrush chromosomes
Obor OECD: Biology (theoretical, mathematical, thermal, cryobiology, biological rhythm), Evolutionary biology
Impakt faktor: 4.096, rok: 2020
Způsob publikování: Open access
https://www.mdpi.com/2073-4425/11/6/617
Interspecific hybridization is a powerful evolutionary force. However, the investigation of hybrids requires the application of methodologies that provide efficient and indubitable identification of both parental subgenomes in hybrid individuals. Repetitive DNA, and especially the satellite DNA sequences (satDNA), can rapidly diverge even between closely related species, hence providing a useful tool for cytogenetic investigations of hybrids. Recent progress in whole-genome sequencing (WGS) offers unprecedented possibilities for the development of new tools for species determination, including identification of species-specific satDNA markers. In this study, we focused on spined loaches (Cobitis, Teleostei), a group of fishes with frequent interspecific hybridization. Using the WGS of one species,C. elongatoides, we identified seven satDNA markers, which were mapped by fluorescence in situ hybridization on mitotic and lampbrush chromosomes ofC. elongatoides,C. taeniaand their triploid hybrids (C. elongatoidesx 2C. taenia). Two of these markers were chromosome-specific in both species, one had centromeric localization in multiple chromosomes and four had variable patterns between tested species. Our study provided a novel set of cytogenetic markers forCobitisspecies and demonstrated that NGS-based development of satDNA cytogenetic markers may provide a very efficient and easy tool for the investigation of hybrid genomes, cell ploidy, and karyotype evolution.
Trvalý link: http://hdl.handle.net/11104/0310325
Počet záznamů: 1