Anaesthesia with diethyl ether impairs jasmonate signalling in the carnivorous plant Venus flytrap (Dionaea muscipula)

0531605 - ÚEB 2021 RIV GB eng J - Článek v odborném periodiku
Pavlovič, A. - Libiaková, M. - Bokor, B. - Jakšová, J. - Petřík, Ivan - Novák, Ondřej - Baluška, F.
Anaesthesia with diethyl ether impairs jasmonate signalling in the carnivorous plant Venus flytrap (Dionaea muscipula).
Annals of Botany. Roč. 125, č. 1 (2020), s. 173-183. ISSN 0305-7364. E-ISSN 1095-8290
Institucionální podpora: RVO:61389030
Klíčová slova: Action potential * Anaesthesia * Anaesthetic * Carnivorous plant * Diethyl ether * Dionaea muscipula * Electrical signal * Jasmonic acid * Plant movement * Venus flytrap
Obor OECD: Plant sciences, botany
Impakt faktor: 4.357, rok: 2020
Způsob publikování: Open access
http://doi.org/10.1093/aob/mcz177

Background and Aims General anaesthetics are compounds that induce loss of responsiveness to environmental stimuli in animals and humans. The primary site of action of general anaesthetics is the nervous system, where anaesthetics inhibit neuronal transmission. Although plants do not have neurons, they generate electrical signals in response to biotic and abiotic stresses. Here, we investigated the effect of the general volatile anaesthetic diethyl ether on the ability to sense potential prey or herbivore attacks in the carnivorous plant Venus flytrap (Dionaea muscipula). Methods We monitored trap movement, electrical signalling, phytohormone accumulation and gene expression in response to the mechanical stimulation of trigger hairs and wounding under diethyl ether treatment. Key Results Diethyl ether completely inhibited the generation of action potentials and trap closing reactions, which were easily and rapidly restored when the anaesthetic was removed. Diethyl ether also inhibited the later response: Jasmonic acid (JA) accumulation and expression of JA-responsive genes (cysteine protease dionain and type I chitinase). However, external application of JA bypassed the inhibited action potentials and restored gene expression under diethyl ether anaesthesia, indicating that downstream reactions from JA are not inhibited. Conclusions The Venus flytrap cannot sense prey or a herbivore attack under diethyl ether treatment caused by inhibited action potentials, and the JA signalling pathway as a consequence.
Trvalý link: http://hdl.handle.net/11104/0310237