A Comparison of Protein and mRNA Expression during Development of the Soil Dwelling Prokaryote (S. coelicolor)

0531246 - MBÚ 2021 RIV US eng J - Článek v odborném periodiku
Pospíšil, Jiří - Strunin, Dmytro - Ziková, Alice - Hubálek, Martin - Vohradský, Jiří
A Comparison of Protein and mRNA Expression during Development of the Soil Dwelling Prokaryote (S. coelicolor).
Proteomics. Roč. 20, č. 14 (2020), č. článku 2000032. ISSN 1615-9853. E-ISSN 1615-9861
Grant CEP: GA MŠMT(CZ) LM2015055; GA MŠMT(CZ) LM2015047; GA MŠMT(CZ) LM2018131
Výzkumná infrastruktura: C4SYS - 90055; ELIXIR-CZ - 90047; ELIXIR-CZ II - 90131
Institucionální podpora: RVO:61388971 ; RVO:61388963
Klíčová slova: computational modeling * mass spectrometry * mRNA
Obor OECD: Biology (theoretical, mathematical, thermal, cryobiology, biological rhythm), Evolutionary biology; Analytical chemistry (UOCHB-X)
Impakt faktor: 3.984, rok: 2020
Způsob publikování: Open access
https://onlinelibrary.wiley.com/doi/abs/10.1002/pmic.202000032

In this paper, correlation analysis of protein and mRNA levels in the soil dwelling bacteriaStreptomyces coelicolor(S. coelicolorM145) is presented during development of the population as it grew in liquid medium using three biological and two technical replicates, measured during exponential growth, and its entry into the stationary phase. The proteome synthesis time series are compared with the gene expression time series measured previously under identical experimental conditions. Results reveal that about one third of protein/mRNA synthesis profiles are well correlated while another third are correlated negatively. Functional analysis of the highly correlated groups is presented. Based on numerical simulation, the negative correlation between protein and mRNA is shown to be caused by the difference between the rate of translation and protein degradation.
Trvalý link: http://hdl.handle.net/11104/0310042