Chlorophyll a fluorescence and Raman spectroscopy can monitor activation/deactivation of photosynthesis and carotenoids in Antarctic lichens

0531212 - ÚVGZ 2021 RIV NL eng J - Článek v odborném periodiku
Mishra, Kumud - Vítek, Petr - Mishra, Anamika - Hajek, J. - Barták, M.
Chlorophyll a fluorescence and Raman spectroscopy can monitor activation/deactivation of photosynthesis and carotenoids in Antarctic lichens.
Spectrochimica Acta Part A-Molecular and Biomolecular Spectroscopy. Roč. 239, OCT (2020), č. článku 118458. ISSN 1386-1425. E-ISSN 1873-3557
Grant CEP: GA MŠMT(CZ) EF16_019/0000797
Výzkumná infrastruktura: CzeCOS III - 90123
Institucionální podpora: RVO:86652079
Klíčová slova: hydration * plants * performance * cryptogams * scytonemin * diversity * granite * ecology * history * growth
Obor OECD: Biophysics
Impakt faktor: 4.098, rok: 2020
Způsob publikování: Omezený přístup
https://www.sciencedirect.com/science/article/pii/S1386142520304364?via%3Dihub

Lichens survive harsh weather of Antarctica as well as of other hostile environments worldwide. Therefore, thisinvestigation isimportantto understand the evolution of lifeon Earth inrelation to their stresstolerance strategy.Wehave used chlorophyllafluorescence (ChlF)and Raman spectroscopy,respectively,to monitorthe activation/deactivation of photosynthesis and carotenoids in three diverse Antarctic lichens,Dermatocarpon polyphyllizum(DP),Umbilicaria antarctica(UA), andLeptogium puberulum(LP). These lichens, post 4 h or 24 h of hydration,showed differences in their ChlF transients and values of major ChlF parameters, e.g., in the maximum quantumefficiency of PSII photochemistry (Fv/Fm), and yields offluorescence and heat dissipation (Φf,d), of effective quan-tum efficiency of PSII photochemistry (ΦPSII) and of non-photochemical quenching (Φnpq), which may be due toquantitative and/or qualitative differences in the composition of their photobionts. For understanding the kinet-ics of hydration-induced activation of photosynthesis, we screenedΦPSIIof these lichens and reported its non-linear stimulation on a minute time scale, half of the activation time (t1/2) was fastest ~4.05 ± 0.29 min for DP,which was followed by 5.46 ± 0.18 min for UA, and 13.95 ± 1.24 min for LP. Upon drying of fully activated lichenthallus, there was a slow decay, in hours, of relative water content (RWC) as well as ofFv/Fm. Raman spectral sig-natures were different for lichens having algal (in DP and UA) and cyanobacteria (in LP) photobionts, and therewas a significant shift inν1(C=C) Raman band of carotenoids post 24 h hydration as compared to their value at adry state or post 4 h of hydration, this shift was decreased, when drying, in DP and LP but not in UA. We concludethat hydration nonlinearly activated photosynthetic apparatus/reactions of these lichens in minute time rangebut there was ade-novosynthesis of chlorophylls as well as of carotenoids post 24 h. Their dehydration-induced deactivation, however, was comparatively slow, in hours range, and there seemed a degradation of syn-thesized chlorophylls and carotenoids post dryness. We conclude that in extremophilic lichens, their photosyn-thetic partners, in particular, possess a complex survival and photoprotective strategy to be successful in theextreme terrestrial environments in Antarctica.
Trvalý link: http://hdl.handle.net/11104/0309927