Analysis of HPV-Positive and HPV-Negative Head and Neck Squamous Cell Carcinomas and Paired Normal Mucosae Reveals Cyclin D1 Deregulation and Compensatory Effect of Cyclin D2

0525100 - ÚMG 2021 RIV CH eng J - Článek v odborném periodiku
Novotný, Jiří - Bandurova, V. - Strnad, Hynek - Chovanec, M. - Hradilová, Miluše - Šáchová, Jana - Steffl, M. - Grušanovič, Josipa - Kodet, R. - Pačes, Václav - Lacina, L. - Smetana, K. , Jr. - Plzák, J. - Kolář, Michal - Vomastek, Tomáš
Analysis of HPV-Positive and HPV-Negative Head and Neck Squamous Cell Carcinomas and Paired Normal Mucosae Reveals Cyclin D1 Deregulation and Compensatory Effect of Cyclin D2.
Cancers (Basel). Roč. 12, č. 4 (2020), č. článku 792. E-ISSN 2072-6694
Grant CEP: GA ČR GA18-11908S; GA MZd(CZ) NV16-29032A; GA MŠMT(CZ) EF16_019/0000785
Institucionální podpora: RVO:68378050 ; RVO:61388971
Klíčová slova: human papillomavirus * head and neck squamous cell carcinoma * cell cycle * D-type cyclins * ccnd1 * ccnd2 * ccnd3 * patient survival * paired tumor-normal samples * 11q13 amplification
Obor OECD: Biochemistry and molecular biology; Oncology (MBU-M)
Impakt faktor: 6.639, rok: 2020
Způsob publikování: Open access
https://www.mdpi.com/2072-6694/12/4/792

Aberrant regulation of the cell cycle is a typical feature of all forms of cancer. In head and neck squamous cell carcinoma (HNSCC), it is often associated with the overexpression of cyclin D1 (CCND1). However, it remains unclear how CCND1 expression changes between tumor and normal tissues and whether human papillomavirus (HPV) affects differential CCND1 expression. Here, we evaluated the expression of D-type cyclins in a cohort of 94 HNSCC patients of which 82 were subjected to whole genome expression profiling of primary tumors and paired normal mucosa. Comparative analysis of paired samples showed that CCND1 was upregulated in 18% of HNSCC tumors. Counterintuitively, CCND1 was downregulated in 23% of carcinomas, more frequently in HPV-positive samples. There was no correlation between the change in D-type cyclin expression and patient survival. Intriguingly, among the tumors with downregulated CCND1, one-third showed an increase in cyclin D2 (CCND2) expression. On the other hand, one-third of tumors with upregulated CCND1 showed a decrease in CCND2. Collectively, we have shown that CCND1 was frequently downregulated in HNSCC tumors. Furthermore, regardless of the HPV status, our data suggested that a change in CCND1 expression was alleviated by a compensatory change in CCND2 expression.
Trvalý link: http://hdl.handle.net/11104/0309315