Simulation-based optimisation of thermodynamic conditions in the ESEM for dynamical in-situ study of spherical polyelectrolyte complex particles in their native state

0525096 - ÚPT 2021 RIV NL eng J - Článek v odborném periodiku
Neděla, Vilém - Tihlaříková, Eva - Maxa, Jiří - Imrichová, Kamila - Bučko, M. - Gemeiner, P.
Simulation-based optimisation of thermodynamic conditions in the ESEM for dynamical in-situ study of spherical polyelectrolyte complex particles in their native state.
Ultramicroscopy. Roč. 211, APR (2020), č. článku 112954. ISSN 0304-3991. E-ISSN 1879-2723
Grant CEP: GA ČR(CZ) GA19-03909S
Institucionální podpora: RVO:68081731
Klíčová slova: ESEM * spherical PEC Particles * MC simulations * thermodynamics * ANSYS * Geant4
Obor OECD: Thermodynamics
Impakt faktor: 2.689, rok: 2020
Způsob publikování: Omezený přístup
https://www.sciencedirect.com/science/article/abs/pii/S0304399119303298

We present a complex analysis and optimisation of dynamic conditions in the environmental scanning electron microscope (ESEM) to allow in-situ observation of extremely delicate wet bio-polymeric spherical particles in their native state. According to the results of gas flow and heat transfer simulations, we were able to develop an improved procedure leading to thermodynamic equilibrium between the sample and chamber environment. To quantify and hence minimise the extent of any sample deformation during specimen chamber pumping, a strength-stress analysis is used. Monte Carlo simulations of beam-gas,water, andsample interactions describe beam scattering, absorbed energy, interaction volume and the emission of signal electrons in the ESEM. Finally, we discuss sample damage as a result of drying and the production of beam-induced free radicals. Based on all experimental and simulation results we introduce a Delicate Sample Observation Strategy for the ESEM. We show how this strategy can be applied to the characterization of polyelectrolyte complex spherical particles containing immobilized recombinant cells E. coli overexpressing cyclohexanone monooxygenase, used as a model biocatalyst. We present the first native-state electron microscopy images of the viscous core of a halved polyelectrolyte complex capsule containing living cells.
Trvalý link: http://hdl.handle.net/11104/0309309