Evolutionarily ancient BAH–PHD protein mediates Polycomb silencing

0525074 - ÚOCHB 2021 RIV US eng J - Článek v odborném periodiku
Wiles, E. T. - McNaught, K. J. - Kaur, G. - Selker, J. M. L. - Ormsby, Tereza - Aravind, L. - Selker, E. U.
Evolutionarily ancient BAH–PHD protein mediates Polycomb silencing.
Proceedings of the National Academy of Sciences of the United States of America. Roč. 117, č. 21 (2020), s. 11614-11623. ISSN 0027-8424. E-ISSN 1091-6490
Institucionální podpora: RVO:61388963
Klíčová slova: epigenetics * facultative heterochromatin * H3K27 methylation * Polycomb repressive complex * histone reader
Obor OECD: Biochemistry and molecular biology
Impakt faktor: 11.205, rok: 2020
Způsob publikování: Omezený přístup
https://www.pnas.org/content/117/21/11614

Methylation of histone H3 lysine 27 (H3K27) is widely recognized as a transcriptionally repressive chromatin modification but the mechanism of repression remains unclear. We devised and implemented a forward genetic scheme to identify factors required for H3K27 methylation-mediated silencing in the filamentous fungus Neurospora crassa and identified a bromo-adjacent homology (BAH)-plant homeodomain (PHD)-containing protein, EPR-1 (effector of polycomb repression 1, NCU07505). EPR-1 associates with H3K27-methylated chromatin, and loss of EPR-1 de-represses H3K27-methylated genes without loss of H3K27 methylation. EPR-1 is not fungal-specific, orthologs of EPR-1 are present in a diverse array of eukaryotic lineages, suggesting an ancestral EPR-1 was a component of a primitive Polycomb repression pathway.
Trvalý link: http://hdl.handle.net/11104/0309292