An appropriate method for assessing hydrogel pore sizes by cryo-sem

0524886 - ÚPT 2021 RIV CZ eng C - Konferenční příspěvek (zahraniční konf.)
Adámková, Kateřina - Trudicová, M. - Hrubanová, Kamila - Sedláček, P. - Krzyžánek, Vladislav
An appropriate method for assessing hydrogel pore sizes by cryo-sem.
10th Anniversary International Conference on Nanomaterials - Research and Application (NANOCON 2018). Ostrava: Tanger, 2019, s. 415-420. ISBN 978-80-87294-89-5.
[Anniversary International Conference on Nanomaterials - Research and Application (NANOCON) /10./. Brno (CZ), 17.10.2018-19.10.2018]
Grant CEP: GA ČR GA17-15451S; GA MŠMT(CZ) LO1212; GA MŠMT ED0017/01/01
Institucionální podpora: RVO:68081731
Klíčová slova: hydrogelcryo-SEM * agarose * cryo-SEM * freezing methods * image analysis
Obor OECD: Polymer science

The aim of our work was to examine and describe ultrastructure of the agarose hydrogel and any possible structural concentration dependencies, and to assess the distribution and size of pores of agarose hydrogel in dependence on its concentration. Four concentrations were prepared (0.5 %, 1.0 %, 2.0 % and 4.0 % of dry weight content) and cryo-SEM and turbidimetry methods were executed on wet (original) samples in order to image the ultrastructure and measure the pore sizes within.
Reasonable results were obtained for the wet samples as they were closer to their native state they are usually used for applications in. Cryo-SEM and turbidimetry provided comparable results of pore diameters and allowed to compare pore diameters dependant on the concentrations, moreover, it showed more detailed and realistic structure.
Trvalý link: http://hdl.handle.net/11104/0309123