The torpedo effect in Bacillus subtilis: RNase J1 resolves stalled transcription complexes

0524333 - MBÚ 2020 RIV US eng J - Článek v odborném periodiku
Šiková, Michaela - Wiedermannová, Jana - Převorovský, M. - Barvík, I. - Sudzinová, Petra - Kofroňová, Olga - Benada, Oldřich - Šanderová, Hana - Condon, C. - Krásný, Libor
The torpedo effect in Bacillus subtilis: RNase J1 resolves stalled transcription complexes.
EMBO Journal. Roč. 39, č. 3 (2020), č. článku e102500. ISSN 0261-4189. E-ISSN 1460-2075
Grant CEP: GA ČR(CZ) GBP305/12/G034; GA ČR(CZ) GA19-12956S; GA MŠMT(CZ) LM2015055; GA MŠMT(CZ) LM2015062; GA MŠMT(CZ) LO1509
Institucionální podpora: RVO:61388971
Klíčová slova: RNAP * RNase J1 * torpedo
Obor OECD: Microbiology
Impakt faktor: 11.598, rok: 2020
Způsob publikování: Open access
https://www.embopress.org/doi/abs/10.15252/embj.2019102500

RNase J1 is the major 5 '-to-3 ' bacterial exoribonuclease. We demonstrate that in its absence, RNA polymerases (RNAPs) are redistributed on DNA, with increased RNAP occupancy on some genes without a parallel increase in transcriptional output. This suggests that some of these RNAPs represent stalled, non-transcribing complexes. We show that RNase J1 is able to resolve these stalled RNAP complexes by a ´torpedo´ mechanism, whereby RNase J1 degrades the nascent RNA and causes the transcription complex to disassemble upon collision with RNAP. A heterologous enzyme, yeast Xrn1 (5 '-to-3 ' exonuclease), is less efficient than RNase J1 in resolving stalled Bacillus subtilis RNAP, suggesting that the effect is RNase-specific. Our results thus reveal a novel general principle, whereby an RNase can participate in genome-wide surveillance of stalled RNAP complexes, preventing potentially deleterious transcription-replication collisions.
Trvalý link: http://hdl.handle.net/11104/0308703