0524215 - ÚFCH JH 2021 RIV CH eng J - Článek v odborném periodiku
Čierna, M. - Naumowicz, M. - Birošová, L. - Krahulec, J. - Sokolová, Romana - Kolivoška, Viliam - Sebechlebská, Táňa - Kielar, F. - Gál, M.
Study of permeabilization of bacterial membrane by electrochemical methods.
Journal of Electroanalytical Chemistry. Roč. 857, JAN 2020 (2020), č. článku 113761. ISSN 1572-6657. E-ISSN 1873-2569
Grant CEP: GA ČR(CZ) GA18-09848S
Institucionální podpora: RVO:61388955
Klíčová slova: antimicrobial peptides * escherichia-coli * carbon nutrition * evolution * ll-37 * Voltammetry * Cathelicidin * Permeabilization * E. coli
Obor OECD: Electrochemistry (dry cells, batteries, fuel cells, corrosion metals, electrolysis)
Impakt faktor: 4.464, rok: 2020 ; AIS: 0.597, rok: 2020
Způsob publikování: Open access
DOI: https://doi.org/10.1016/j.jelechem.2019.113761

Selected electrochemical methods for the determination of possible membrane permeabilization in two Escherichia coli bacterial strains were examined. Escherichia coli, a gram-negative rod-shaped bacterium, is an important component of the mammalian intestinal microbiota. However, some strains of this bacterium are pathogenic and can cause a variety of diseases. Partial aim of this work was to identify beta-galactosidase enzyme as one of the possible factors characterizing disintegration of bacterial membrane. To permeabilize the bacterial membrane, cathelicidin LL-37, the body's own antimicrobial peptide, and membrane degradation by microwave radiation were used. In the investigation of structural changes in the bacterial membrane, square-wave voltammetry and chronopotentiometric dissolution analysis in combination with the mercury drop electrode and the glassy carbon electrode were used. UV/VIS spectrophotometry was used to indirectly track changes in bacterial cell structure. (C) 2019 The Authors. Published by Elsevier B.V.
Trvalý link: http://hdl.handle.net/11104/0308594