“Clickable” and antifouling block copolymer brushes as a versatile platform for peptide‐specific cell attachment

0523814 - ÚMCH 2021 RIV DE eng J - Článek v odborném periodiku
Poreba, Rafal - de los Santos Pereira, Andres - Pola, Robert - Jiang, S. - Pop-Georgievski, Ognen - Sedláková, Zdeňka - Schönherr, H.
“Clickable” and antifouling block copolymer brushes as a versatile platform for peptide‐specific cell attachment.
Macromolecular Bioscience. Roč. 20, č. 4 (2020), s. 1-10, č. článku 1900354. ISSN 1616-5187. E-ISSN 1616-5195
Grant CEP: GA MŠMT(CZ) LQ1604; GA ČR(CZ) GA19-02739S
Grant ostatní: AV ČR(CZ) MSM200501701
Program: Program na podporu mezinárodní spolupráce začínajících výzkumných pracovníků
Institucionální podpora: RVO:61389013
Klíčová slova: antifouling * copolymer brushes * controlled-cell attachment
Obor OECD: Polymer science
Impakt faktor: 4.979, rok: 2020
Způsob publikování: Open access
https://onlinelibrary.wiley.com/doi/full/10.1002/mabi.201900354

To tailor cell–surface interactions, precise and controlled attachment of cell‐adhesive motifs is required, while any background non‐specific cell and protein adhesion has to be blocked effectively. Herein, a versatile and highly reproducible antifouling surface modification based on “clickable” groups and hierarchically structured diblock copolymer brushes for the controlled attachment of cells is reported. The polymer brush architecture combines an antifouling bottom block of poly(2‐hydroxyethyl methacrylate) poly(HEMA) and an ultrathin azide‐bearing top block, which can participate in well‐established “click” reactions including the highly selective copper‐catalyzed alkyne‐azide cycloaddition (CuAAC) reaction under mild conditions. This straightforward approach allows the rapid conjugation of a cell‐adhesive, alkyne‐bearing cyclic RGD peptide motif, enabling subsequent specific attachment of NIH 3T3 fibroblasts, their extensive proliferation and confluent cell sheet formation after 48 h of incubation. The generally applicable strategy presented in this report can be employed for surface functionalization with diverse alkyne‐bearing biological moieties via CuAAC or copper‐free alkyne‐azide cycloaddition protocols, making it a versatile functionalization approach and a promising tool for tissue engineering, biomaterial implant design, and other applications that require surfaces supporting highly specific cell attachment.
Trvalý link: http://hdl.handle.net/11104/0308248