Introduction to RNAi and miRNA pathways

0523492 - ÚMG 2020 RIV CZ eng M - Část monografie knihy
Svoboda, Petr
RNAi and miRNA pathways in plants I - molecular mechanisms.
Introduction to RNAi and miRNA pathways. Praha: Karolinum, 2019 - (Svoboda, P.), s. 1-339. ISBN 978-80-246-4372-4
Grant CEP: GA MŠMT LO1220
Institucionální podpora: RVO:68378050
Klíčová slova: dsRNA * siRNA * miRNA * Dicer * Argonaute * RdRP * RNAi * PTGS * DNA methylation
Obor OECD: Biology (theoretical, mathematical, thermal, cryobiology, biological rhythm), Evolutionary biology

RNA silencing denotes sequence-specific repression mediated by small RNAs. It includes post-transcriptional silencing mechanisms such as RNA interference (RNAi) and microRNA (miRNA) pathway or transcriptional silencing. RNA silencing regulates endogenous protein-coding gene expression, serves as a form of innate immunity targeting viruses, and protects genome integrity by repression of retrotransposons. This review provides overview of RNA silencing in flowering plants, from which come most published data. Plants have arguably the most complex RNA silencing system among eukaryots because of existence of many paralogs of key protein factors, which form in an intricate network of primary and secondary small RNAs, which mediate transcriptional and post-transcriptional effects. The first half of the review will provide a detailed catalogue of components of RNA silencing in plants while the second half will discuss the specific silencing mechanisms themselves and their biological roles, with a particular focus on the miRNA pathway, PTGS/VIG (plant’s equivalent of RNAi), and transcriptional gene silencing (TGS), which is a unique RNA silencing adaptation existing in plants.

Trvalý link: http://hdl.handle.net/11104/0307855