0523265 - BTÚ 2020 RIV eng U - Conference, Workshop Arrangement
Kubista, Mikael - Šindelka, Radek - Valihrach, Lukáš - Švec, David - Rohlová, Eva - Smolík, Ondřej - Franko, Filip
TATAA courses – 3 days Hands-on qPCR.
[Vestec, 08.04.2019-10.04.2019, (W-WRD 9/5)]
R&D Projects: GA MŠMT(CZ) ED1.1.00/02.0109
Institutional support: RVO:86652036
Keywords : qPCR * biostatistic * reverese transcription
OECD category: Biochemistry and molecular biology

Day 1. Introduction to PCR and qPCR. qPCR data evaluation. Primer and probe design. Optimization of qPCR protocols. Hands-on lab running qPCR analyzing unknown samples with standard curve. Day 2. Normalization. Basic quantification theory. Absolute quantification strategies. Validation of assays. Quantification calculation examples. Hands-on lab running qPCR and calculating relative quantities with different strategies. Day 3. Sample preparation and isolation. Reverse transcription, RT. The MIQE guidelines. Group discussion
Permanent Link: http://hdl.handle.net/11104/0307633