Advances in Disease Models

0523206 - ÚMG 2020 RIV CZ eng M - Část monografie knihy
Hrčkulák, Dušan - Kořínek, Vladimír
Intestinal organoid cultures – methodology for isolation, propagation, and freezing of mouse intestinal epithelium in vitro.
Advances in Disease Models. Praha: OPTIO CZ, 2019 - (Bartůněk, P.), s. 1-310. ISBN 978-80-88011-06-4
Grant CEP: GA MŠMT LO1419
Institucionální podpora: RVO:68378050
Klíčová slova: organoid cultures * methodology for isolation * mouse intestinal epithelium
Obor OECD: Cell biology

Intestinal organoid cultures represent a suitable model for studying the organisation and homeostasis of the intestinal epithelium in vitro. Here we present a step-by-step protocol for establishing and propagating organoid cultures from the freshly isolated mouse small intestine and colon. We provide detailed information related to materials and workflow based on the original procedure that has been modified according to our experience.
Trvalý link: http://hdl.handle.net/11104/0307584