Monocytes undergo multi-step differentiation in mice during oral infection by Toxoplasma gondii

0523095 - BTÚ 2020 RIV GB eng J - Článek v odborném periodiku
Detavernier, C. - Azouz, A. - Shehade, H. - Splittgerber, M. - Van Maele, L. - Nguyen, M. - Thomas, S. - Achouri, Y. - Švec, David - Calonne, E. - Fuks, F. - Oldenhove, G. - Goriely, S.
Monocytes undergo multi-step differentiation in mice during oral infection by Toxoplasma gondii.
Communications Biology. Roč. 2, č. 1 (2019), č. článku 472. E-ISSN 2399-3642
Grant CEP: GA ČR(CZ) GJ18-24753Y; GA MŠMT(CZ) ED1.1.00/02.0109
Institucionální podpora: RVO:86652036
Klíčová slova: dendritic cells * inflammatory monocytes * activation * reveals * macrophages * expression
Obor OECD: Other biological topics
Impakt faktor: 4.165, rok: 2019
Způsob publikování: Open access
https://www.nature.com/articles/s42003-019-0718-6.pdf

Monocytes play a major role in the defense against pathogens. They are rapidly mobilized to inflamed sites where they exert both proinflammatory and regulatory effector functions. It is still poorly understood how this dynamic and exceptionally plastic system is controlled at the molecular level. Herein, we evaluated the differentiation process that occurs in Ly6C(hi) monocytes during oral infection by Toxoplasma gondii. Flow cytometry and single-cell analysis revealed distinct activation status and gene expression profiles in the bone marrow, the spleen and the lamina propria of infected mice. We provide further evidence that acquisition of effector functions, such as the capacity to produce interleukin-27, is accompanied by distinct waves of epigenetic programming, highlighting a role for STAT1/IRF1 in the bone marrow and AP-1/NF-kappa B in the periphery. This work broadens our understanding of the molecular events that occur in vivo during monocyte differentiation in response to inflammatory cues.
Trvalý link: http://hdl.handle.net/11104/0307500