Osteoinductive 3D scaffolds prepared by blend centrifugal spinning for long-term delivery of osteogenic supplements

0522983 - ÚEM 2020 RIV GB eng J - Článek v odborném periodiku
Lukášová, Věra - Buzgo, M. - Vocetková, Karolína - Kubíková, T. - Tonar, Z. - Doupnik, M. - Hefka Blahnová, Veronika - Litvinec, Andrej - Sovková, Věra - Voltrová, Barbora
Osteoinductive 3D scaffolds prepared by blend centrifugal spinning for long-term delivery of osteogenic supplements.
RSC Advances. Roč. 8, č. 39 (2018), s. 21889-21904. E-ISSN 2046-2069
Grant CEP: GA ČR(CZ) GA15-15697S; GA ČR(CZ) GA16-14758S; GA MŠMT(CZ) LO1309; GA MŠMT(CZ) LO1508
Institucionální podpora: RVO:68378041
Klíčová slova: mesenchymal stem-cells * marrow stromal cells * drug-delivery
Obor OECD: Biomaterials (as related to medical implants, devices, sensors)
Impakt faktor: 3.049, rok: 2018
Způsob publikování: Open access
https://pubs.rsc.org/en/content/articlelanding/2018/RA/C8RA02735H#!divAbstract

Bone regeneration is a long-term process requiring proper scaffolding and drug delivery systems. The current study delivers a three-dimensional (3D) scaffold prepared by blend centrifugal spinning loaded with the osteogenic supplements (OS) -glycerol phosphate, ascorbate-2-phosphate and dexamethasone. The OS were successfully encapsulated into a fibrous scaffold and showed sustained release for 30 days. Furthermore, biological testing showed the osteoinductive properties of the scaffolds on a model of human mesenchymal stem cells and stimulatory effect on a model of osteoblasts. The osteoinductive properties were further proved in vivo in critical size defects of rabbits. The amount of bone trabecules was bigger compared to control fibers without OS. The results indicate that due to its long-term drug releasing properties, single step fabrication process and 3D structure, the system shows ideal properties for use as a cell-free bone implant in tissue-engineering.
Trvalý link: http://hdl.handle.net/11104/0307391