Pairing of homologous chromosomes in C. elegans meiosis requires DEB-1-an orthologue of mammalian vinculin

0522832 - ÚMG 2020 RIV US eng J - Článek v odborném periodiku
Rohožková, Jana - Hůlková, Lenka - Fukalová, Jana - Flachs, Petr - Hozák, Pavel
Pairing of homologous chromosomes in C. elegans meiosis requires DEB-1-an orthologue of mammalian vinculin.
Nucleus. Roč. 10, č. 1 (2019), s. 93-115. ISSN 1949-1034. E-ISSN 1949-1042
Grant CEP: GA ČR GA16-03403S; GA ČR(CZ) GA18-19714S; GA MŠMT LTC17054; GA MŠMT(CZ) LM2015062
Grant ostatní: GA MŠk(CZ) CZ.1.05/1.1.00/02.0109
Institucionální podpora: RVO:68378050
Klíčová slova: deb-1 * LINC complex * chromosome pairing * prophase I * c * elegans * vinculin
Obor OECD: Cell biology
Impakt faktor: 2.792, rok: 2019
Způsob publikování: Open access
https://www.tandfonline.com/doi/full/10.1080/19491034.2019.1602337

During meiosis, homologous chromosomes undergo a dramatic movement in order to correctly align. This is a critical meiotic event but the molecular properties of this 'chromosomal dance' still remainunclear. We identified DEB-1 an orthologue of mammalian vinculin as a new component of the mechanistic modules responsible for attaching the chromosomes to the nuclear envelope as apart of the LINC complex. In early meiotic nuclei of C. elegans, DEB-1 is localized to the nuclear periphery and alongside the synaptonemal complex of paired homologues. Upon DEB-1 depletion, chromosomes attached to SUN-1 foci remain highly motile until late pachytene. Although the initiation of homologue pairing started normally, irregularities in the formation of the synaptonemal complex occur, and these results in meiotic defects such as increased number of univalents at diakinesis and high embryonic lethality. Our data identify DEB-1 as a new player regulating chromosome dynamics and pairing during meiotic prophase I.
Trvalý link: http://hdl.handle.net/11104/0307257