Precise CRISPR/Cas9 Editing of the NHE1 Gene Renders Chickens Resistant to the J Subgroup of Avian Leukosis Virus

0521805 - ÚMG 2021 RIV US eng J - Článek v odborném periodiku
Koslová, Anna - Trefil, P. - Mucksová, J. - Reinišová, Markéta - Plachý, Jiří - Kalina, J. - Kučerová, Dana - Geryk, Josef - Krchlíková, Veronika - Lejčková, B. - Hejnar, Jiří
Precise CRISPR/Cas9 Editing of the NHE1 Gene Renders Chickens Resistant to the J Subgroup of Avian Leukosis Virus.
Proceedings of the National Academy of Sciences of the United States of America. Roč. 117, č. 4 (2020), s. 2108-2012. ISSN 0027-8424. E-ISSN 1091-6490
Grant CEP: GA ČR GA15-23993S; GA MŠMT LO1419; GA MZe(CZ) QK1810344
Institucionální podpora: RVO:68378050
Klíčová slova: CRISPR/Cas9 genome editing in chicken * Na+/H+ exchanger type 1 * avian leukosis virus subgroup J * disease resilience in poultry * primordial germ cells
Obor OECD: Virology
Impakt faktor: 11.205, rok: 2020
Způsob publikování: Open access
https://www.pnas.org/content/117/4/2108

Avian leukosis virus subgroup J (ALV-J) is an important concern for the poultry industry. Replication of ALV-J depends on a functional cellular receptor, the chicken Na+/H+ exchanger type 1 (chNHE1). Tryptophan residue number 38 of chNHE1 (W38) in the extracellular portion of this molecule is a critical amino acid for virus entry. We describe a CRISPR/Cas9-mediated deletion of W38 in chicken primordial germ cells and the successful production of the gene-edited birds. The resistance to ALV-J was examined both in vitro and in vivo, and the ΔW38 homozygous chickens tested ALV-J-resistant, in contrast to ΔW38 heterozygotes and wild-type birds, which were ALV-J-susceptible. Deletion of W38 did not manifest any visible side effect. Our data clearly demonstrate the antiviral resistance conferred by precise CRISPR/Cas9 gene editing in the chicken. Furthermore, our highly efficient CRISPR/Cas9 gene editing in primordial germ cells represents a substantial addition to genotechnology in the chicken, an important food source and research model.
Trvalý link: http://hdl.handle.net/11104/0306370