Transmembrane adaptor protein WBP1L regulates CXCR4 signalling and murine haematopoiesis

0521715 - ÚMG 2020 RIV US eng J - Článek v odborném periodiku
Borna, Šimon - Drobek, Aleš - Králová, Jarmila - Glatzová, Daniela - Šplíchalová, Iva - Fabišik, Matěj - Pokorná, Jana - Skopcová, Tereza - Angelisová, Pavla - Kanderová, V. - Starková, J. - Stanek, P. - Matveichuk, O. - Pavliuchenko, Nataliia - Kwiatkowska, K. - Protty, M. B. - Tomlinson, M. G. - Alberich-Jorda, Meritxell - Kořínek, Vladimír - Brdička, Tomáš
Transmembrane adaptor protein WBP1L regulates CXCR4 signalling and murine haematopoiesis.
Journal of Cellular and Molecular Medicine. Roč. 24, č. 2 (2020), s. 1980-1992. ISSN 1582-1838. E-ISSN 1582-4934
Grant CEP: GA MŠMT(CZ) ED1.1.00/02.0109; GA MŠMT LO1419; GA ČR GA16-07425S; GA ČR(CZ) GBP302/12/G101; GA MŠMT(CZ) LM2015040; GA MŠMT(CZ) LM2015062; GA MŠMT ED2.1.00/19.0395
Institucionální podpora: RVO:68378050
Klíčová slova: bone marrow homing * bone marrow transplantation * cxcr4 * etv6 * haematopoiesis * haematopoietic stem cell * NEDD4 family * opal1 * runx1 * wbp1l
Obor OECD: Cell biology
Impakt faktor: 5.310, rok: 2020
Způsob publikování: Open access
https://onlinelibrary.wiley.com/doi/full/10.1111/jcmm.14895

WW domain binding protein 1-like (WBP1L), also known as outcome predictor of acute leukaemia 1 (OPAL1), is a transmembrane adaptor protein, expression of which correlates with ETV6-RUNX1 (t(12,21)(p13,q22)) translocation and favourable prognosis in childhood leukaemia. It has a broad expression pattern in haematopoietic and in non-haematopoietic cells. However, its physiological function has been unknown. Here, we show that WBP1L negatively regulates signalling through a critical chemokine receptor CXCR4 in multiple leucocyte subsets and cell lines. We also show that WBP1L interacts with NEDD4-family ubiquitin ligases and regulates CXCR4 ubiquitination and expression. Moreover, analysis of Wbp1l-deficient mice revealed alterations in B cell development and enhanced efficiency of bone marrow cell transplantation. Collectively, our data show that WBP1L is a novel regulator of CXCR4 signalling and haematopoiesis.
Trvalý link: http://hdl.handle.net/11104/0306301