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Hit detection in serial femtosecond crystallography using X-ray spectroscopy of plasma emission
- 1.0521655 - FZÚ 2020 RIV GB eng J - Článek v odborném periodiku
Jonsson, H.O. - Caleman, C. - Andreasson, Jakob - Timneanu, N.
Hit detection in serial femtosecond crystallography using X-ray spectroscopy of plasma emission.
IUCrJ. Roč. 4, Nov (2017), s. 778-784. ISSN 2052-2525. E-ISSN 2052-2525
Grant CEP: GA MŠMT EF15_008/0000162
Grant ostatní: ELI Beamlines(XE) CZ.02.1.01/0.0/0.0/15_008/0000162
Institucionální podpora: RVO:68378271
Klíčová slova: hit detection * plasma emission spectra * serial femtosecond crystallography * protein structure
Obor OECD: Fluids and plasma physics (including surface physics)
Impakt faktor: 6.544, rok: 2017
Způsob publikování: Open access
The upcoming X-ray laser sources will produce well above 1000 pulses per second and will pose a new challenge: how to quickly determine successful crystal hits and avoid a high-rate data deluge. Proposed here is a hit-finding scheme based on detecting photons from plasma emission after the sample has been intercepted by the X-ray laser. Plasma emission spectra are simulated for systems exposed to high-intensity femtosecond pulses, for both protein crystals and the liquid carrier systems that are used for sample delivery. The thermal radiation from the glowing plasma gives a strong background in the XUV region that depends on the intensity of the pulse, around the emission lines from light elements (carbon, nitrogen, oxygen).
Trvalý link: http://hdl.handle.net/11104/0306244
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