Počet záznamů: 1
Electrospray sample injection for single-particle imaging with x-ray lasers
- 1.0521634 - FZÚ 2020 RIV US eng J - Článek v odborném periodiku
Bielecki, J. - Hantke, M.F. - Daurer, B.J. - Reddy, H.K.N. - Hasse, D. - Larsson, D.S.D. - Gunn, L.H. - Svenda, M. - Munke, A. - Sellberg, J.A. - Flueckiger, L. - Pietrini, A. - Nettelblad, C. - Lundholm, I. - Carlsson, G. - Okamoto, K. - Timneanu, N. - Westphal, D. - Kulyk, Olena - Higashiura, A. - van der Schot, G. - Loh, N.-T.D. - Wysong, T.E. - Bostedt, C. - Gorkhover, T. - Iwan, A. - Seibert, M.M. - Osipov, T. - Walter, P. - Hart, Ph. - Bucher, M. - Ulmer, A. - Ray, D. - Carini, G. - Ferguson, K.R. - Andersson, I. - Andreasson, Jakob - Hajdu, Janos - Maia, F.R.N.C.
Electrospray sample injection for single-particle imaging with x-ray lasers.
Science Advances. Roč. 5, č. 5 (2019), s. 1-9, č. článku eaav8801. ISSN 2375-2548. E-ISSN 2375-2548
Grant CEP: GA MŠMT LQ1606
Institucionální podpora: RVO:68378271
Klíčová slova: mass-spectrometry * crystallography * generation * mobility
Obor OECD: Fluids and plasma physics (including surface physics)
Impakt faktor: 13.117, rok: 2019
Způsob publikování: Open access
The possibility of imaging single proteins constitutes an exciting challenge for x-ray lasers. Despite encouraging results on large particles, imaging small particles has proven to be difficult for two reasons: not quite high enough pulse intensity from currently available x-ray lasers and, as we demonstrate here, contamination of the aerosolized molecules by nonvolatile contaminants in the solution. The amount of contamination on the sample depends on the initial droplet size during aerosolization. Here, we show that, with our electrospray injector, we can decrease the size of aerosol droplets and demonstrate virtually contaminant-free sample delivery of organelles, small virions, and proteins. The results presented here, together with the increased performance of next-generation x-ray lasers, constitute an important stepping stone toward the ultimate goal of protein structure determination from imaging at room temperature and high temporal resolution.
Trvalý link: http://hdl.handle.net/11104/0306226
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