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Nuclear pore protein TPR associates with lamin B1 and affects nuclear lamina organization and nuclear pore distribution
- 1.0521614 - ÚMG 2020 RIV CH eng J - Článek v odborném periodiku
Fišerová, Jindřiška - Maninová, Miloslava - Sieger, T. - Uhlířová, Jana - Šebestová, Lenka - Efenberková, Michaela - Čapek, Martin - Fišer, K. - Hozák, Pavel
Nuclear pore protein TPR associates with lamin B1 and affects nuclear lamina organization and nuclear pore distribution.
Cellular and Molecular Life Sciences. Roč. 76, č. 11 (2019), s. 2199-2216. ISSN 1420-682X. E-ISSN 1420-9071
Grant CEP: GA ČR GJ15-08835Y; GA ČR(CZ) GA16-03346S; GA ČR GA16-03403S; GA ČR(CZ) GA17-09103S; GA ČR(CZ) GA18-19714S; GA MŠMT(CZ) ED1.1.00/02.0109; GA MŠMT(CZ) LM2015062; GA MŠMT LO1419
Institucionální podpora: RVO:68378050
Klíčová slova: Translocated promoter region * tpr * Lamina * Lamins * Nuclear pore complex * Super-resolution imaging * Image analysis * Nucleus
Obor OECD: Cell biology
Impakt faktor: 6.496, rok: 2019
Způsob publikování: Omezený přístup
https://link.springer.com/article/10.1007/s00018-019-03037-0
The organization of the nuclear periphery is crucial for many nuclear functions. Nuclear lamins form dense network at the nuclear periphery and play a substantial role in chromatin organization, transcription regulation and in organization of nuclear pore complexes (NPCs). Here, we show that TPR, the protein located preferentially within the nuclear baskets of NPCs, associates with lamin B1. The depletion of TPR affects the organization of lamin B1 but not lamin A/C within the nuclear lamina as shown by stimulated emission depletion microscopy. Finally, reduction of TPR affects the distribution of NPCs within the nuclear envelope and the effect can be reversed by simultaneous knock-down of lamin A/C or the overexpression of lamin B1. Our work suggests a novel role for the TPR at the nuclear periphery: the TPR contributes to the organization of the nuclear lamina and in cooperation with lamins guards the interphase assembly of nuclear pore complexes.
Trvalý link: http://hdl.handle.net/11104/0306213
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