Brassinosteroids induce strong, dose-dependent inhibition of etiolated pea seedling growth correlated with ethylene production

0521079 - ÚEB 2020 RIV CH eng J - Článek v odborném periodiku
Jiroutová, Petra - Mikulík, Jaromír - Novák, Ondřej - Strnad, Miroslav - Oklešťková, Jana
Brassinosteroids induce strong, dose-dependent inhibition of etiolated pea seedling growth correlated with ethylene production.
Biomolecules. Roč. 9, č. 12 (2019), č. článku 849. E-ISSN 2218-273X
Grant CEP: GA MŠMT(CZ) EF16_019/0000827
Institucionální podpora: RVO:61389030
Klíčová slova: brassinosteroid * growth inhibition * bioassay
Obor OECD: Biochemistry and molecular biology
Impakt faktor: 4.082, rok: 2019
Způsob publikování: Open access
http://dx.doi.org/10.3390/biom9120849

We have recently discovered that brassinosteroids (BRs) can inhibit the growth of etiolated pea seedlings dose-dependently in a similar manner to the 'triple response' induced by ethylene. We demonstrate here that the growth inhibition of etiolated pea shoots strongly correlates with increases in ethylene production, which also responds dose-dependently to applied BRs. We assessed the biological activities of two natural BRs on pea seedlings, which are excellent material as they grow rapidly, and respond both linearly and uni-phasically to applied BRs. We then compared the BRs' inhibitory effects on growth, and induction of ethylene and ACC (1-aminocyclopropane-1-carboxylic acid) production, to those of representatives of other phytohormone classes (cytokinins, auxins, and gibberellins). Auxin induced ca. 50-fold weaker responses in etiolated pea seedlings than brassinolide, and the other phytohormones induced much weaker (or opposite) responses. Following the optimization of conditions for determining ethylene production after BR treatment, we found a positive correlation between BR bioactivity and ethylene production. Finally, we optimized conditions for pea growth responses and developed a new, highly sensitive, and convenient bioassay for BR activity.
Trvalý link: http://hdl.handle.net/11104/0305744