Advanced impedimetric biosensor configuration and assay protocol for glycoprofiling of a prostate oncomarker using Au nanoshells with a magnetic core

0520765 - FZÚ 2020 RIV NL eng J - Článek v odborném periodiku
Bertók, T. - Lorencova, L. - Hroncekova, S. - Gajdosova, V. - Jane, E. - Hires, M. - Kasák, P. - Kaman, Ondřej - Sokol, R. - Bella, V. - Eckstein, A.A. - Mosnáček, J. - Vikartovská, A. - Tkac, J.
Advanced impedimetric biosensor configuration and assay protocol for glycoprofiling of a prostate oncomarker using Au nanoshells with a magnetic core.
Biosensors and Bioelectronics. Roč. 131, Apr (2019), s. 24-29. ISSN 0956-5663. E-ISSN 1873-4235
Grant CEP: GA ČR GA16-04340S
Institucionální podpora: RVO:68378271
Klíčová slova: Au nanoshells with a magnetic core * diazonium salt * carboxybetaine * prostate specific antigen * impedance spectroscopy * cancer biomarker
Obor OECD: Condensed matter physics (including formerly solid state physics, supercond.)
Impakt faktor: 10.257, rok: 2019
Způsob publikování: Omezený přístup
https://doi.org/10.1016/j.bios.2019.01.052

In this paper several advances were implemented for glycoprofiling of prostate specific antigen (PSA), what can be applied for better prostate cancer (PCa) diagnostics in the future: 1) application of Au nanoshells with a magnetic core (MP@silica@Au): 2) use of surface plasmons of Au nanoshells with a magnetic core for spontaneous immobilization of zwitterionic molecules via diazonium salt grafting, 3) a double anti-fouling strategy with integration of zwitterionic molecules on Au surface and on MP@silica@Au particles was implemented to resist non-specific protein binding, 4) application of anti-PSA antibody modified Au nanoshells with a magnetic core for enrichment of PSA from a complex matrix of a human serum, 5) direct incubation of anti-PSA modified MP@silica@Au with affinity bound PSA to the lectin modified electrode surface.
Trvalý link: http://hdl.handle.net/11104/0305436