Early Detection on Pancreatic Cancer: 1H NMR Metabolomics Study of its Connection with Diabetes Mellitus.

0520609 - ÚCHP 2020 PT eng C - Konferenční příspěvek (zahraniční konf.)
Michálková, Lenka - Horník, Štěpán - Habartová, L. - Sýkora, Jan - Setnička, V.
Early Detection on Pancreatic Cancer: 1H NMR Metabolomics Study of its Connection with Diabetes Mellitus.
Conference Program. Porto: SMASH NMR Conference, 2019, s. 115-116, č. článku 34. ISBN N.
[Small Molecular NMR Conference 2019 (SMASH 2019). Porto (PT), 22.09.2019-25.09.2019]
Institucionální podpora: RVO:67985858
Klíčová slova: diabetes mellitus * nmr spectroscopy * statistical analysis
Obor OECD: Medical engineering

In this work, we tested the strategy for the detection of T3cDM and early PC based on NMR metabolomics. ’H NMR spectroscopy was used to detect changes in the concentration of low molecular metabolites in the blood plasma of four groups: PC patients, long-term T2DM (lasting more than 5 years), new-onset diabetic patients (lasting less than 3 years, potential T3cDM) and healthy controls. The ChenomX software was used to identify 65 metabolites across all samples. Based on the concentrations of the identified metabolites, methods of pattern recognition (primarily PCA and OPLS-DA) and statistical tests (t-test and Wilcoxon rank sum test) were used to differentiate between the studied groups. The obtained results suggested a panel of biomarkers for the detection of PC. Within the group of new-onset diabetics, our proposed OPLS- DA model, (verified by Monte Carlo cross-validation), was able to predict possible T3cDM patients at risk of developing PC. Regarding such results, the suggested model might serve as a screening method for the early detection of PC.
Trvalý link: http://hdl.handle.net/11104/0305253