Morphological, Ultrastructural, Motility and Evolutionary Characterization of Two New Hemistasiidae Species

0520426 - BC 2020 RIV DE eng J - Článek v odborném periodiku
Prokopchuk, Galina - Tashyreva, Daria - Yabuki, A. - Horák, Aleš - Masařová, Petra - Lukeš, Julius
Morphological, Ultrastructural, Motility and Evolutionary Characterization of Two New Hemistasiidae Species.
Protist. Roč. 170, č. 3 (2019), s. 259-282. ISSN 1434-4610. E-ISSN 1618-0941
Grant CEP: GA ČR(CZ) GA16-18699S; GA ČR(CZ) GA18-23787S; GA MŠMT(CZ) LL1601; GA MŠMT(CZ) EF16_019/0000759; GA MŠMT(CZ) LM2015062; GA MŠMT(CZ) EF16_013/0001775
Institucionální podpora: RVO:60077344
Klíčová slova: diplonema-ambulator larsen * flagellates protista * sequence alignment * phylogeny * marine * patterson * redescription * euglenozoon * homologies * colorless * Diplonemids * Hemistasiidae * rickettsiid * endosymbiosis * extrusome
Obor OECD: Genetics and heredity (medical genetics to be 3)
Impakt faktor: 1.989, rok: 2019
Způsob publikování: Omezený přístup
https://www.sciencedirect.com/science/article/abs/pii/S1434461018301391?via%3Dihub

Until now, Hemistasia phaeocysticola was the only representative of the monogeneric family Hemistasiidae available in culture. Here we describe two new axenized hemistasiids isolated from Tokyo Bay, Japan. Like in other diplonemids, cellular organization of these heterotrophic protists is characterized by a distinct apical papilla, a tubular cytopharynx contiguous with a deep flagellar pocket, and a highly branched mitochondrion with lamellar cristae. Both hemistasiids also bear a prominent digestive vacuole, peripheral lacunae, and paraflagellar rods, are highly motile and exhibit diverse morphologies in culture. We argue that significant differences in molecular phylogenetics and ultrastructure between these new species and H. phaeocysticola are on the generic level. Therefore, we have established two new genera within Hemistasiidae Artemidia gen. n. and Namystynia gen. n. to accommodate Artemidia motanka, sp. n. and Namystynia karyoxenos, sp. n., respectively. A. motanka permanently carries tubular extrusomes, while in N. karyoxenos, they are present only in starving cells. An additional remarkable feature of the latter species is the presence, in both the cytoplasm and the nucleus, of the endosymbiotic rickettsiid Candidatus Sneabacter namystus. (C) 2019 Elsevier GmbH. All rights reserved.
Trvalý link: http://hdl.handle.net/11104/0305102