Počet záznamů: 1
Tracking of Borrelia afzelii Transmission from Infected Ixodes ricinus Nymphs to Mice
- 1.0520425 - BC 2020 RIV US eng J - Článek v odborném periodiku
Pospíšilová, Tereza - Urbanová, Veronika - Hes, O. - Kopáček, Petr - Hajdušek, Ondřej - Šíma, Radek
Tracking of Borrelia afzelii Transmission from Infected Ixodes ricinus Nymphs to Mice.
Infection and Immunity. Roč. 87, č. 6 (2019), č. článku e00896-18. ISSN 0019-9567. E-ISSN 1098-5522
Grant CEP: GA ČR(CZ) GA17-27393S; GA ČR(CZ) GA17-27386S; GA ČR(CZ) GA18-01832S; GA MŠMT(CZ) EF16_019/0000759
GRANT EU: European Commission(XE) 602272 - ANTIDotE
Institucionální podpora: RVO:60077344
Klíčová slova: lyme-disease spirochete * burgdorferi sensu-stricto * gene-expression * north-america * ticks * ospc * time * kinetics * protein * growth * Borrelia * Borrelia afzelii * Ixodes ricinus * Lyme disease * tick-borne pathogens * transmission
Obor OECD: Microbiology
Impakt faktor: 3.201, rok: 2019
Způsob publikování: Open access
https://iai.asm.org/content/iai/87/6/e00896-18.full.pdf
Quantitative and microscopic tracking of Borrelia afzelii transmission from infected Ixodes ricinus nymphs has shown a transmission cycle different from that of Borrelia burgdorferi and Ixodes scapularis. Borrelia afzelii organisms are abundant in the guts of unfed I. ricinus nymphs, and their numbers continuously decrease during feeding. Borrelia afzelii spirochetes are present in murine skin within 1 day of tick attachment. In contrast, spirochetes were not detectable in salivary glands at any stage of tick feeding. Further experiments demonstrated that tick saliva is not essential for B. afzelii infectivity, the most important requirement for successful host colonization being a change in expression of outer surface proteins that occurs in the tick gut during feeding. Spirochetes in vertebrate mode are then able to survive within the host even in the absence of tick saliva. Taken together, our data suggest that the tick gut is the decisive organ that determines the competence of I. ricinus to vector B. afzelii. We discuss possible transmission mechanisms of B. afzelii spirochetes that should be further tested in order to design effective preventive and therapeutic strategies against Lyme disease.
Trvalý link: http://hdl.handle.net/11104/0305100
Počet záznamů: 1