Metabolomics: Methods and Protocols

0520369 - MBÚ 2020 RIV US eng M - Část monografie knihy
Pluháček, Tomáš - Škríba, Anton - Novák, Jiří - Luptáková, Dominika - Havlíček, Vladimír
Analysis of Microbial Siderophores by Mass Spectrometry.
Metabolomics: Methods and Protocols. New York: HUMANA PRESS INC, 2019 - (Bhattacharya, S.), s. 131-153. ISBN 978-1-4939-9488-5
Grant CEP: GA MŠMT(CZ) LO1509; GA ČR(CZ) GA19-10907S
Institucionální podpora: RVO:61388971
Klíčová slova: Siderophore * Mass spectrometry * Extraction
Obor OECD: Analytical chemistry

Siderophores represent important microbial virulence factors and infection biomarkers. Their monitoring in fermentation broths, bodily fluids, and tissues should be reproducible. Similar isolation, characterization, and quantitation studies can often have conflicting results, and without proper documentation of sample collection, data processing, and analysis methods, it is difficult to reexamine the data and reconcile these differences. In this Springer Nature Protocol, we present the procedure optimized for ferricrocin/triacetylfusarinine C extraction from biological material as well as for tissue fixation and cryosectioning for optical microscopy and for both elemental and molecular mass spectrometry imaging. Special attention is paid to siderophore data mining from conventional and product ion mass spectra, liquid chromatography, and mass spectrometry imaging datasets, performed here by our free software called CycloBranch.
Trvalý link: http://hdl.handle.net/11104/0305048