Measuring venous-arterial differences of valine, isoleucine, leucine, alanine and glutamine in skeletal muscles using counter-current electrophoresis with contactless conductivity detection

0519392 - ÚOCHB 2021 RIV CH eng J - Článek v odborném periodiku
Tůma, P. - Gojda, J. - Sommerová, B. - Koval, Dušan
Measuring venous-arterial differences of valine, isoleucine, leucine, alanine and glutamine in skeletal muscles using counter-current electrophoresis with contactless conductivity detection.
Journal of Electroanalytical Chemistry. Roč. 857, Jan 15 (2020), č. článku 113772. ISSN 1572-6657. E-ISSN 1873-2569
Grant CEP: GA ČR(CZ) GA17-12648S
Institucionální podpora: RVO:61388963
Klíčová slova: branched chain amino acids * clinical analysis * capillary electrophoresis * coating * contactless conductivity detection
Obor OECD: Analytical chemistry
Impakt faktor: 4.464, rok: 2020
Způsob publikování: Omezený přístup
https://www.sciencedirect.com/science/article/pii/S1572665719310409?via%3Dihub

Plasmatic levels of valine, isoleucine, leucine, alanine and glutamine were determined by capillary electrophoresis with contactless conductivity detection. A new kind of covalently coated capillary based on polyacrylamide and (3-acrylamidopropyl) trimethylammonium chloride was employed. The amino acids were separated in the counter-current regime, in which the analytes migrate under counter-current electro-osmotic flow with a value of 5.53 · 10−9 m2 V−1 s−1 in the background electrolyte, 3.2 mol/L AcOH +10% v/v MeOH. The baseline separation of all five amino acids from other plasmatic compounds was achieved in <5 min, the limits of detections are 0.13–0.14 μmol/L for the treated sample and 0.50–0.58 μmol/L for the untreated plasma. The method was used to measure venous-arterial differences of amino acids in hyperinsulinemic patients with cachexia.
Trvalý link: http://hdl.handle.net/11104/0304510