0518840 - ÚFE 2020 DE eng A - Abstrakt
Marín, Antonio García - Holanová, Kristýna - Bujak, Lukasz - Vala, Milan - Piliarik, Marek
Scattering labels for ultrafast tracking of biomolecular machinery.
European Biophysics Journal With Biophysics Letters. Roč. 48, Supplement: 1 (2019), S186-S186. ISSN 0175-7571. E-ISSN 1432-1017.
[Joint 12th EBSA European Biophysics Congress / 10th IUPAP International Conference on Biological Physics (ICBP). 20.07.2019-24.07.2019, Madrid]
Institucionální podpora: RVO:67985882
Obor OECD: Biophysics

The study of the function of biological structures has attracted a great deal of attention in the last years. In that respect, tracking single-protein behavior by means of labels and localization microscopy has been established as the method of choice, improving our understanding of life at the single-molecule level. One of the promising tools for this purpose is the interferometric scattering microscopy (iSCAT), which relies on scattering labels for creating strong contrasts from the background signal, via elastic scattering processes [1,2]. Among all the possible scatterers, gold nanoparticles (GNPs) have shown a great potential thanks to their ease of synthesis and well-known surface chemistry, especially relevant for the conjugation of the GNP with a certain biomolecule (e.g. proteins or DNA strands) [3]. These surface functionalization processes typically rely on the chemisorption of highly-specific molecules, known as a linker, to further anchor the target biomolecule selectively on the GNP surface through covalent bonds. However, some applications may require a strict control in the amount of linkers on the GNP surface to avoid cross-linking effects or other possible sources of uncertainty in the measurement. Optimization of this process is, thus, a critical part to obtain reliable results. In this work, we focus on the preparation and optimization of GNPs for tracking different fast biological events in order to achieve a nanometer precision with a microsecond time resolutions. The applicability of these GNPs is demonstrated by tracking the microtubule disassembly and the movement of microtubule-associated proteins, such as PCR1
Trvalý link: http://hdl.handle.net/11104/0303870