The processes associated with lipid peroxidation in human embryonic lung fibroblasts, treated with polycyclic aromatic hydrocarbons and organic extract from particulate matter

0518532 - ÚEM 2020 RIV GB eng J - Článek v odborném periodiku
Rössner ml., Pavel - Líbalová, Helena - Červená, Tereza - Vrbová, Kristýna - Elzeinova, Fatima - Milcová, Alena - Rössnerová, Andrea - Nováková, Zuzana - Ciganek, M. - Pokorná, Michaela
The processes associated with lipid peroxidation in human embryonic lung fibroblasts, treated with polycyclic aromatic hydrocarbons and organic extract from particulate matter.
Mutagenesis. Roč. 34, č. 2 (2019), s. 153-164. ISSN 0267-8357. E-ISSN 1464-3804
Grant CEP: GA ČR(CZ) GA16-14631S
Institucionální podpora: RVO:68378041
Klíčová slova: NF-kappa-B * oxidative stress * damage
Obor OECD: Toxicology
Impakt faktor: 3.379, rok: 2019
Způsob publikování: Open access s časovým embargem
https://academic.oup.com/mutage/article-abstract/34/2/153/5372984?redirectedFrom=fulltext

Polycyclic aromatic hydrocarbons (PAHs) may cause lipid peroxidation via reactive oxygen species generation. 15-F-2t-isoprostane (IsoP), an oxidative stress marker, is formed from arachidonic acid (AA) by a free-radical induced oxidation. AA may also be converted to prostaglandins (PG) by prostaglandin-endoperoxide synthase (PTGS) induced by NF-kappa B. We treated human embryonic lung fibroblasts (HEL12469) with benzo[a]pyrene (B[a]P), 3-nitrobenzanthrone (3-NBA) and extractable organic matter (EOM) from ambient air particulate matter <2.5 mu m for 4 and 24 h. B[a]P and 3-NBA induced expression of PAH metabolising, but not antioxidant enzymes. The concentrations of IsoP decreased, whereas the levels of AA tended to increase. Although the activity of NF-kappa B was not detected, the tested compounds affected the expression of prostaglandin-endoperoxide synthase 2 (PTGS2). The levels of prostaglandin E-2 (PGE(2)) decreased following exposure to B[a]P, whereas 3-NBA exposure tended to increase PGE(2) concentration. A distinct response was observed after EOM exposure: expression of PAH-metabolising enzymes was induced, IsoP levels increased after 24-h treatment but AA concentration was not affected. The activity of NF-kappa B increased after both exposure periods, and a significant induction of PTGS2 expression was found following 4-h treatment. Similarly to PAHs, the EOM exposure was associated with a decrease of PGE(2) levels. In summary, exposure to PAHs with low pro-oxidant potential results in a decrease of IsoP levels implying 'antioxidant' properties. For such compounds, IsoP may not be a suitable marker of lipid peroxidation.
Trvalý link: http://hdl.handle.net/11104/0303652